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05-1579

Anti-Axin-1 Antibody, clone A5

clone A5, from mouse

Synonym(s):

Axis inhibition protein 1, axin 1, axis inhibitor 1, fused, mouse, homolog of

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
A5, monoclonal
Application:
ICC, IP, WB
Citations:
6
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biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

A5, monoclonal

species reactivity

human

species reactivity (predicted by homology)

mouse (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NP_033863.2

UniProt accession no.

O35625

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... AXIN1(8312)
mouse ... Axin1(12005)

General description

Axin-1 is constitutively expressed and acts as a negative regulator of Wnt expression. It has been demonstrated that mouse Wnt, Wnt3a, is secreted by multiple cell lines and can act and as a growth factor for stem cells. Secreted Wnt3a is highly hydrophobic because it is palmitoylated on an essential cysteine C77; the palmitoylated state of Wnt3a is essential for stem cell signaling. Incubation of stem cells with Wnt3a in tissue culture medium resulted in the increased number of mouse hematopoietic stem cells expressing "primitive" phenotype.
~ 97 kDa

Immunogen

GST-tagged recombinant protein corresponding to the N-terminus of mouse Axin-1.

Application

Detect Axin-1 using this Anti-Axin-1 Antibody, clone A5 validated for use in WB, IP & IC.

Biochem/physiol Actions

This antibody recognizes the N-terminus of Axin-1.

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blot using immunoprecipitated Axin-1 in SW-480 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Axin-1 immunoprecipitated from 10 µg of SW-480 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Phosphatidylinositol 3-kinase signaling does not activate the wnt cascade.
Ng, SS; Mahmoudi, T; Danenberg, E; Bejaoui, I; de Lau, W; Korswagen, HC; Schutte, M; Clevers, H
The Journal of Biological Chemistry null
Chuifeng Fan et al.
Molecular carcinogenesis, 54 Suppl 1, E138-E147 (2014-09-30)
Our previous studies indicate that abnormal expression of several Wnt signaling molecules including Axin, Dvl and β-catenin are involved in proliferation, invasion and metastasis of lung cancer. Zbed3 was found to inhibit function of Axin-GSK3β complex and thus lead to
Rong Xie et al.
eLife, 11 (2022-12-22)
Axin1 is a key regulator of canonical Wnt signaling pathway. Roles of Axin1 in skeletal development and in disease occurrence have not been fully defined. Here, we report that Axin1 is essential for lower limb development. Specific deletion of Axin1
View All Related Papers

Global Trade Item Number

SKUGTIN
05-157904053252272547