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About This Item
NACRES:
NA.56
UNSPSC Code:
41106500
packaging
pack of 10 mL
manufacturer/tradename
Cytiva 17-0756-01
storage condition
(20% Ethanol )
parameter
<100 cm/hr flow rate (Sample loading), 100—300 cm/hr flow rate (wash and elution)
technique(s)
liquid chromatography (LC): suitable
matrix
4% agarose
matrix active group
glutathione
average diameter
90 μm
cleaning in place
4-13
working range
4-13
capacity
>25 mg binding capacity (recombinant GST / ml)
separation technique
affinity
shipped in
ambient
storage temp.
2-30°C
General description
Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
Glutathione Sepharose™ 4B is designed for high capacity single-step purification of Glutathione S-Transferase (GST) tagged fusion proteins.
GST-tagged proteins and other glutathione S-transferases or glutathone-dependent proteins can be purified directly from bacterial lysates using Glutathione Sepharose™ 4B. Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity.
GST-tagged proteins and other glutathione S-transferases or glutathone-dependent proteins can be purified directly from bacterial lysates using Glutathione Sepharose™ 4B. Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity.
Features and Benefits
- High capacity single step purification of recombinant glutathione S-transferase (GST) tagged proteins
- Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity and functionality.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Sepharose is a trademark of Cytiva
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signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
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