H2387
Hanks′ Balanced Salts
Modified, without calcium chloride, magnesium sulfate and sodium bicarbonate, powder, suitable for cell culture
Synonym(s):
HBSS
form
powder
technique(s)
cell culture | mammalian: suitable
storage temp.
2-8°C
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General description
Balanced salt solution (BSS) provides cells with water, bulk inorganic ions, and buffering system to maintain the normal cell metabolism and physiological range (pH 7.2-7.6), respectively. It also acts as an irrigating, diluting, and transporting agent and maintains the intracellular and extracellular osmotic balance. BSS also provides energy sources such as glucose for cell metabolism.
Application
Hanks′ Balanced Salt has been used:
- to culture primary hippocampal neuron cells from rats
- to collect the rat brains
- to culture rat primary hepatocytes
Preparation Note
Formulated to contain 9.5 grams of powder per liter of medium.
Supplement with 0.35 g/L sodium bicarbonate.
Other Notes
The pH is adjusted after addition of sodium bicarbonate.
Storage Class
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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