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115943 Methylene blue (C.I.52015)

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115943
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Overview

Replacement Information

Key Spec Table

CAS #EC NumberHill FormulaChemical FormulaMolar Mass
61-73-4200-515-2C₁₆H₁₈ClN₃S * x H₂OC₁₆H₁₈ClN₃S * x H₂O (x=2-3)319.86 g/mol (anhydrous)

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      Description
      Catalogue Number115943
      SynonymsMethylthioninium chloride, 3,7-Bis(dimethylamino)phenothiazinium chloride, Tetramethylthionine chloride
      DescriptionMethylene blue (C.I.52015)
      OverviewMethylene blue (C.I. 52015) for microscopy Certistain® is a dry dye that is used in bacteriology for an overview stain and as counterstaining for Ziehl-Neelsen method in sample material of human origin. Bacteria appear blue. The dye has a further important application in hematology: Methylene blue and eosin are the main components of many staining solutions and dye mixtures. Certistain® is the product group for seleceted high quality dyes that fulfill all quality requirements of IVD and CE registration. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
      References
      Product Information
      CAS number61-73-4
      EC number200-515-2
      Hill FormulaC₁₆H₁₈ClN₃S * x H₂O
      Chemical formulaC₁₆H₁₈ClN₃S * x H₂O (x=2-3)
      Molar Mass319.86 g/mol (anhydrous)
      HS Code3212 90 00
      Structure formula ImageStructure formula Image
      Quality LevelMQ400
      Applications
      Applicationfor microscopy Certistain®
      Biological Information
      Physicochemical Information
      Melting Point180 °C (decomposition)
      pH value3 (10 g/l, H₂O, 20 °C)
      Bulk density400 - 600 kg/m3
      Solubility50 g/l
      Dimensions
      Materials Information
      Toxicological Information
      LD 50 oralLD50 Rat 1180 mg/kg
      Safety Information according to GHS
      Hazard Pictogram(s) Hazard Pictogram(s)
      Hazard Statement(s)H302: Harmful if swallowed.
      Precautionary Statement(s)P264: Wash skin thoroughly after handling.
      P270: Do not eat, drink or smoke when using this product.
      P301 + P312: IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell.
      P501: Dispose of contents/ container to an approved waste disposal plant.
      Signal WordWarning
      Storage class10 - 13 Other liquids and solids
      WGKWGK 2 obviously hazardous to water
      Disposal3
      Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
      Safety Information
      Categories of dangerharmful
      Product Usage Statements
      Storage and Shipping Information
      StorageStore at +2°C to +30°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Dye content (spectrophotometrically)≥ 82 %
      Identity
      - Chloride

      passes test
      - UV/VIS-Spectrumpasses test
      Absorption maximum λ max (ethanol 50 %)660 - 665 nm
      Spec. Absorptivity A 1%/1cm (λmax; 0.003 g/l; ethanol 50 %)2250 - 2750
      TLC-Testpasses test
      Loss on drying (110 °C)10 - 15 %
      Suitability for microscopypasses test
      Global Trade Item Number
      Catalogue Number GTIN
      1159430025 04022536162584

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      Categories

      IVD/OEM Materials & Reagents > Microscopy > Dyes for Microscopy > Certistain® Dyes
      Reagents, Chemicals and Labware > Inorganic Reagents > Safety Products and General Applications > Indicators > Redox Indicators