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Merck

1.08488

Urea

for biochemistry

Synonym(s):

Carbamide, Carbonyl diamide, Diaminomethanone, Carbonyldiamine, Carbamide, Carbonyldiamide

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About This Item

Linear Formula:
NH2CONH2
CAS Number:
57-13-6
Molecular Weight:
60.06
UNSPSC Code:
12352111
NACRES:
NA.21
EC Index Number:
200-315-5
MDL number:
MFCD00008022
Beilstein/REAXYS Number:
635724

Product Name

Urea, for biochemistry

InChI key

XSQUKJJJFZCRTK-UHFFFAOYSA-N

SMILES string

NC(N)=O

InChI

1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)

vapor pressure

<0.1 hPa ( 20 °C)

form

solid

potency

8471 mg/kg LD50, oral (Rat)
8200 mg/kg LD50, skin (Rat)

technique(s)

titration: suitable

pH

9 (20 °C, 100 g/L in H2O)

mp

132-135 °C (lit.)

solubility

1000 g/L

density

1.335 g/mL at 25 °C (lit.)

bulk density

720‑760 kg/m3

storage temp.

10-30°C

Quality Level

100

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Analysis Note

Purity (DSC (differential scanning calorimetry)): ≥ 99.5 %
Identity (IR-spectrum): passes test
Appearance: Colorless crystals or white crystalline powder.
Appearance of solution (100 g/l, water): Clear and colorless.
Melting point (DSC (differential scanning calorimetry)): 132 - 135 °C
UV-Absorption (260 nm; 8 mol/l; 1 cm; water): ≤ 0.1
UV-Absorption (280 nm; 8 mol/l; 1 cm; water): ≤ 0.1
Cyanide (CN): ≤ 0.000001 %
Chloride (Cl): ≤ 0.0005 %
Sulfate (SO₄): ≤ 0.001 %
Heavy metals (as Pb): ≤ 0.0004 %
Fe (Iron): ≤ 0.00002 %
NH₄ (Ammonium): ≤ 0.00001 %
Biuret: ≤ 0.05 %
Sulfated ash (800 °C): ≤ 0.01 %

Application

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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