199664
Sudan Black B
certified by the BSC
Synonym(s):
Ceres black BN, Fat Black HB, Solvent Black 3
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About This Item
Empirical Formula (Hill Notation):
C29H24N6
CAS Number:
Molecular Weight:
456.54
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
224-087-1
MDL number:
Beilstein/REAXYS Number:
723248
Colour Index Number:
26150
agency
certified by the BSC
Quality Level
form
powder
technique(s)
microbe id | staining: suitable
color
dark brown to very dark brown
mp
120-124 °C (lit.)
λmax
415 nm (2nd), 598 nm
ε (extinction coefficient)
≥20000 at 596-603 nm in ethanol
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
CC1(C)Nc2cccc3c(ccc(N1)c23)\N=N\c4ccc(\N=N\c5ccccc5)c6ccccc46
InChI
1S/C29H24N6/c1-29(2)30-26-14-8-13-22-25(17-18-27(31-29)28(22)26)35-34-24-16-15-23(20-11-6-7-12-21(20)24)33-32-19-9-4-3-5-10-19/h3-18,30-31H,1-2H3/b33-32+,35-34+
InChI key
YCUVUDODLRLVIC-VPHDGDOJSA-N
Application
Sudan Black B has been used in brain BLAQ (Block Lipids and Aldehyde Quench) technique and is used in lipid detection.
Certified as a fat staining using the Churukian variant of the Lillie-Ashburn supersaturated isopropanol method. Sudan III is substituted for the oil red O in the procedure. Burdon′s stain for bacterial lipid.
Biochem/physiol Actions
Sudan Black B has been used to stain phospholipids and intracellular lipids. Staining pattern is almost similar to myeloperoxidase reaction, with positive staining of granulocytic cells, eosinophils and weak monocytic staining.It doesn′t stain lymphocytes. Sudan Black B can be used to stain older blood or bone marrow smears, but the stain slightly fades over time. It is considered to be a most sensitive and specific reagent for lipid staining. Sudan Black B stains phospholipids gray and neutral fats blue-black.
signalword
Warning
hcodes
Hazard Classifications
Muta. 2
Storage Class
11 - Combustible Solids
wgk
WGK 3
ppe
Eyeshields, Gloves, type N95 (US)
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H N Subramaniam et al.
Journal of biochemical and biophysical methods, 21(1), 9-16 (1990-06-01)
The selective staining of neutral lipids in Human Amnion cells in tissue culture was achieved using a particular fraction of the lipid dye, Sudan black B and a standardized staining procedure. The fraction, termed SBB-I, was isolated by thin-layer chromatography.
Acetylated Sudan Black B as a More Specific Histochemical Reagent for Lipides
CASSELMAN WGB
Journal of Cell Science, 321- 322 (1954)
Brain BLAQ: Post-hoc thick-section histochemistry for localizing optogenetic constructs in neurons and their distal terminals
Kupferschmidt DA
Frontiers in Neuroanatomy (2015)