A8887

Acrylamide

Name: Acrylamide
Brand: SIGMA

suitable for electrophoresis, ≥99%

Synonym(s):

2-Propenamide, Polyacrylamide, acrylic amide, akrylamid, Acrylic acid amide

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About This Item

Linear Formula:

CH₂=CHCONH₂

CAS Number:

79-06-1

Molecular Weight:

71.08

NACRES:

NB.22

PubChem Substance ID:

24891378

eCl@ss:

39031205

UNSPSC Code:

41105319

EC Number:

201-173-7

MDL number:

MFCD00008032

Beilstein/REAXYS Number:

605349

Product Name

Acrylamide, suitable for electrophoresis, ≥99%

InChI key

HRPVXLWXLXDGHG-UHFFFAOYSA-N

InChI

1S/C3H5NO/c1-2-3(4)5/h2H,1H2,(H2,4,5)

SMILES string

NC(=O)C=C

vapor density

2.45 (vs air)

vapor pressure

0.03 mmHg ( 40 °C)

assay

≥99%

form

crystals

technique(s)

electrophoresis: suitable

bp

125 °C/25 mmHg (lit.)

mp

82-86 °C (lit.)

solubility

water: soluble 2.5 g/10 mL, clear, colorless

suitability

suitable for electrophoresis

Quality Level

200

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Application

Acrylamide has been used:

for the synthesis of hydrogels
for the preparation of polyacrylamide gel
to agglomerate vimentin and keratin intermediate filament network

Acrylamide has been used:

to agglomerate the vimentin and keratin intermediate filament network of K562cells
in the preparation and characterization of soft culture substrate
in hydrogel synthesis
for determination of protein by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) electrophoresis

General description

Acrylamide acts as a quencher of tryptophan fluorescence. Acrylamide alkylates SH groups of cysteine and ε-NH2 lysine residues of proteins during electrophoresis. Bis-acrylamide is required for crosslinking and acrylamide is vital for the polymerization of monomers. Acrylamide along with bis-acrylamide crosslinks to form polyacrylamide gels, which is used to separate protein and other biomolecules.

Acrylamide (AA) is a neurotoxin and carcinogen. Potato products like, French fries, potato chips and also several cereal-based products contains acrylamide. This highly reactive organic compound has the ability to polymerize to form polyacrylamide.

pictograms

GHS06,GHS08

signalword

Danger

hcodes

H301,H312 + H332,H315,H317,H319,H340,H350,H361f,H372

pcodes

P202 - P280 - P301 + P310 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

target_organs

Peripheral nervous system

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

280.4 °F - closed cup

flash_point_c

138 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

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Use of potato flour in bread and flat bread

Flour and Breads and Their Fortification in Health and Disease Prevention, 247-259 (2011)

Expression and Ni-NTA-Agarose Purification of Recombinant Hepatitis C Virus E2 Ectodomain Produced in a Baculovirus Expression System

Gomez-Gutirrrez J, et al.

Biochimica et Biophysica Acta - Biomembranes (2018)

A large deformation viscoelastic model for double-network hydrogels

Mao Y, et al.

Journal of the Mechanics and Physics of Solids, 100, 103-130 (2017)

Matrix stiffness modulates formation and activity of neuronal networks of controlled architectures

Lantoine J, et al.

Biomaterials, 89, 14-24 (2016)

Voltage-induced wrinkle performance in a hydrogel by dielectric elastomer actuation

Tang C, et al.

Polymer, 10(7), 697-697 (2018)

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