D225
4,5-Diaminofluorescein diacetate solution
5 mM in DMSO, ≥97% (HPLC)
Synonym(s):
DAF-2 DA
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About This Item
Quality Level
assay
≥97% (HPLC)
form
solution
availability
not available in Japan
concentration
5 mM in DMSO
shipped in
wet ice
storage temp.
−20°C
General description
4,5-Diaminofluorescein diacetate (DAF-2 DA) solution is a membrane-permeable fluorescent probe, which measures nitric oxide (NO) in living cells. It is used to study the synthesis of NO in bovine aortic endothelial cells (BAECs). DAF-2 DA imaging shows that ciliated epithelial cells produces high amount of NO than non-epithelial inflammatory cells.
Application
Cell-permeable fluorescent detector of nitric oxide in living cells.
Legal Information
DAF-2 is manufactured by Daiichi Pure Chemicals Co., LTD.
US Patent No. 5,874,590
US Patent No. 5,874,590
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
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Determinants of shear stress-stimulated endothelial nitric oxide production assessed in real-time by 4, 5-diaminofluorescein fluorescence
Qiu W, et al.
Biochemical and Biophysical Research Communications, 286(2), 328-335 (2001)
Increased nitric oxide production in nasal epithelial cells from allergic patients-RT-PCR analysis and direct imaging by a fluorescence indicator: DAF-2 DA
Takeno S, et al.
Clinical and Experimental Allergy, 31(6), 881-888 (2001)
Rachel A Battaglia et al.
eLife, 8 (2019-11-05)
Alexander disease (AxD) is a fatal neurodegenerative disorder caused by mutations in glial fibrillary acidic protein (GFAP), which supports the structural integrity of astrocytes. Over 70 GFAP missense mutations cause AxD, but the mechanism linking different mutations to disease-relevant phenotypes