MES hydrate

MES hydrate has been used:

• in the MS (Murashige and Skoog) media for growth of Arabidopsis seedlings.
• in the cytoskeleton buffer during immunofluorescence study.
• as a wash buffer to wash the paramagnetic beads and resuspend them for binding mannose-BSA
• as a component of growth medium

MES hydrate, or 4-morpholineethanesulfonic acid hydrate, is a zwitterionic and morpholinic buffer renowned as one of the "Good′s" buffer for biological and biochemical applications. Its outstanding buffering capacity in the pH range of 5.5 to 6.7 makes it versatile for a wide array of research applications. Notably, MES Buffer minimizes interaction with common metals used in environmental and biological studies, preventing interference with experimental outcomes.

With excellent water solubility and virtual insolubility in lipids, MES Buffer is impermeable to membranes, ensuring efficacy in cellular environments. Its applications span maintaining stability in cell culture media, protein-based buffer formulations, and electrophoresis running buffers. MES Buffer may also find usage in purifying antibodies, peptides, proteins, blood components, and growth factors. MES Buffer stands out as a better alternative to potentially toxic agents like cacodylate and non-zwitterionic buffers such as citrate and malate. MES Buffer proves valuable for adjusting growth media pH, stabilizing enzymatic solutions, and serving as a component of PAGE running buffer in diverse experimental settings.

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A buffer using MES can be prepared by titrating with NaOH to the desired pH. Alternatively, stock solutions of MES and MES sodium salt can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare buffers of a given pH have been published. MES is not recommended for buffering at pH 7.4; other buffers should be considered.

Solutions are stable at 2-8°C for months. Sterilize by filtration through 0.2uM filters. Autoclaving is not recommended for any sulfonic acid buffer. If buffers must be nuclease-free, treat the water first, and then add the buffer after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown.