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Merck

P4682

Phenol solution

Saturated with 0.01 M citrate buffer, pH 4.3 ± 0.2, BioReagent, Molecular Biology

Synonym(s):

nucleic acid extraction

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About This Item

Empirical Formula (Hill Notation):
C6H6O
CAS Number:
108-95-2
Molecular Weight:
94.11
UNSPSC Code:
12352104
NACRES:
NA.52
PubChem Substance ID:
57654523
MDL number:
MFCD00002143
Beilstein/REAXYS Number:
6428463
grade:
Molecular Biology
technique(s):
RNA purification: suitable

Product Name

Phenol solution, Saturated with 0.01 M citrate buffer, pH 4.3 ± 0.2, BioReagent, Molecular Biology

InChI

1S/C6H6O/c7-6-4-2-1-3-5-6/h1-5,7H

SMILES string

Oc1ccccc1

InChI key

ISWSIDIOOBJBQZ-UHFFFAOYSA-N

grade

Molecular Biology

product line

BioReagent

form

liquid

technique(s)

RNA purification: suitable

pH

4.1-4.5 (25 °C)

storage temp.

2-8°C

Quality Level

200

Gene Information

human ... GABRA1(2554)

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Application

Phenol solution has been used:

  • for the extraction of RNA by standard phenol-chloroform protocols
  • in cell wall monosaccharide analysis to treat epidermal strips from the abaxial side of Commelina communisin leaves
  • as a component of lysis-denaturation buffer for RNA isolation

Biochem/physiol Actions

Phenol solution can be used for a wide variety of molecular biology applications, including nucleic acid extractions using standard phenol-chloroform protocols. This organic solvent also serves as a potential protein denaturant. Phenol-chloroform extraction is a broadly used method for the extraction of nucleic acids. Despite phenol′s incomplete inhibition of RNase activity, it possesses characteristics that make it useful for isolating nucleic acids when combined with chloroform and alcohol.

General description

Phenol solution is a clear liquid saturated with citrate buffer. Phenols are organic compounds that feature a hydroxyl group directly bonded to an aromatic carbocyclic ring. The common name for monohydroxybenzene is phenol.

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

target_organs

Nervous system,Kidney,Liver,Skin

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
MKCZ9480
MKCZ3435
MKCX5024
MKCX5490
MKCX5440

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PHENOLS
Smith PWG and Tatchell AR
Aromatic Chemistry, 79-104 (1969)
Sagit Shalel-Levanon et al.
Biotechnology and bioengineering, 92(2), 147-159 (2005-07-01)
Escherichia coli has several elaborate sensing mechanisms for response to the availability of oxygen and the presence of other electron acceptors. The adaptive responses are coordinated by a group of global regulators, which include the one-component Fnr protein, and the
Effect of oxygen, and ArcA and FNR regulators on the expression of genes related to the electron transfer chain and the TCA cycle in Escherichia coli
Levanon SS, et al.
Metabolic engineering, 7(5-6), 364-374 (2005)
mRNA expression profiles for Escherichia coli ingested by normal and phagocyte oxidase-deficient human neutrophils
Staudinger BJ, et al.
The Journal of Clinical Investigation, 110(8), 1151-1163 (2002)
Nucleic Acid Extraction Techniques
Shin JH, et al.
Journal of Separation Science, 209?225-209?225 (2012)
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