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Merck

PEROX1

Peroxisome Isolation Kit

isolate peroxisomes from tissues and cells

Synonym(s):

Isolation Kit for Peroxisomes, Kit for Peroxisomes, Peroxisome Kit

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352200
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Quality Level

technique(s)

centrifugation: suitable, fractionation: suitable

shipped in

wet ice

storage temp.

2-8°C

General description

Isolated peroxisomes are used for studying lipid β-oxidation, amino acid metabolism and biosynthesis of ether-linked glycerolipids and bile acids.

Application

The Perixosome Isolation Kit provides all the necessary reagents and a detailed protocol for the isolation of highly purified peroxisomes from animal tissues and cells, by differential density gradient centrifugation using iodixanol [OptiPrep]. This kit has been used for preparation of peroxisomes from rat liver, rat kidney and rabbit liver as well as HEK293 and HepG2 cells.

Features and Benefits

  • Specially formulated extraction reagents for research scale applications - save time and minimize waste
  • Produces functional intact organelles - resulting peroxisomes are suitable for functional studies, metabolic assays, protein profiling, and disease state analysis
  • Compatible with products for structure confirmation - easily confirm intactness with companion test kit, Cytochrome C Reductase Assay Kit (Cat. No. CY0100)

Other Notes

Upon receiving the kit, the Protease Inhibitor Cocktail (Product Code P 8340) should be stored at –20 °C and the OptiPrep Density Gradient Medium (Product Code O3028) should be stored at room temperature.

Legal Information

OptiPrep is a trademark of Serumwerk Bernburg AG


Kit Components Also Available Separately

Product No.
Description
SDS & Pricing

  • Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution
    SDS

pictograms

Corrosion

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

wgk

WGK 2

Storage Class

10 - Combustible liquids



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Shurong Hou et al.
SLAS discovery : advancing life sciences R & D, 22(7), 887-896 (2017-03-28)
Primary hyperoxaluria is the underlying cause of oxalosis and is a life-threatening autosomal recessive disease, for which treatment may require dialysis or dual liver-kidney transplantation. The most common primary hyperoxaluria type 1 (PH1) is caused by genetic mutations of a
C M Rodrigues et al.
Journal of lipid research, 37(3), 540-550 (1996-03-01)
We recently demonstrated that the formation of delta 22-bile acids is a quantitatively major pathway for normal bile acid synthesis in the adult male Sprague-Dawley rat. This pathway is specific for 7 beta-hydroxy bile acids and, when ursodeoxycholic acid is
K Burdett et al.
The Journal of biological chemistry, 266(19), 12201-12206 (1991-07-05)
Upon differential centrifugation of guinea pig intestine mucosal cells homogenate, fatty acyl-CoA:NADPH oxidoreductase (long chain alcohol forming) was found to be enriched in the light mitochondrial (L) fraction (sedimenting between 66,000 x g min and 500,000 x g min) which