T8154
Trypan Blue solution
0.4%, liquid, sterile-filtered, suitable for cell culture
Synonym(s):
Blue cell viability dye
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About This Item
Product Name
Trypan Blue solution, 0.4%, liquid, sterile-filtered, suitable for cell culture
form
liquid
InChI
1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
SMILES string
[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
InChI key
GLNADSQYFUSGOU-UHFFFAOYSA-J
sterility
sterile-filtered
storage condition
dry at room temperature
concentration
0.4%
technique(s)
cell culture | mammalian: suitable
tissue processing: suitable
application(s)
cell analysis
shipped in
ambient
Quality Level
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Related Categories
Application
- in cell viability assay to count viable and dead cells
- in rescue assay to count viable cells
- in trypan blue exclusion counting method to determine proliferation curve
- as the saline control and in the preparation of lipopolysaccharide (LPS) solution to confirm the success of LPS infusion
- in trypan blue dye exclusion test to determine gastric epithelial cells (GEC) viability
- to count GEC cells using a hemocytometer
General description
Trypan Blue (TB), an anionic hydrophilic azo dye, crosses only the cell membranes of dead cells, thereby staining dead tissues/cells blue. It is used in quantitative microscopy for a dye exclusion staining technique to differentiate between live and dead mammalian cells.
Preparation Note
signalword
Danger
hcodes
Hazard Classifications
Carc. 1B
Storage Class
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
Do the cells need to be washed to remove the culture media prior to staining with Product T8154, Trypan Blue solution?
Trypan Blue has a greater affinity for serum proteins than for cellular protein. If the background is too dark, cells should be pelleted and resuspended in protein-free medium or salt solution prior to counting.
Why is Trypan blue, Product T8154, used to determine cell viability?
Trypan Blue is a vital stain recommended for use in estimating the proportion of viable cells in a population. The reactivity of this dye is based on the fact that the chromophore is negatively charged and does not react with the cell unless the membrane is damaged. Staining facilitates the visualization of cell morphology. Live (viable) cells do not take up the dye and dead (non-viable) cells do.
Can Trypan blue, Product T8154, be used to discriminate between apoptotic and necrotic cells?
Trypan blue will stain cells that have disrupted membranes. It cannot differentiate between permeabilized membranes caused by apoptosis or necrosis.
Trypan blue, Product T8154, contains a precipitate. Is it still good to use?
The trypan blue solution is still good to use. It can be warmed to dissolve the precipitate or it can be filtered to remove the precipitate.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Why are there red fibers in Product T8154, Trypan blue solution?
A report of fibers in Product T8154, Trypan blue solution is sufficiently common almost to be a characteristic of the product. These fibers are a cosmetic fault rather than one of functionality. The presence of fibers may be more pronounced following cold storage or shipping. This product should be stored at room temperature. Filtration to remove the fibers will not affect the use of the product.
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