Protein delivery system

Protein delivery system, IGF-1, PODS contains the polyhedrin protein co-crystalized with Human IGF-1. Insulin-like Growth Factor 1 (IGF-1) is a growth factor that is produced by the liver. IGF-1 production is stimulated by Growth Hormone. IGF-1 binds the insulin-like growth factor 1 receptor (IGF1R) and the insulin receptor to stimulate systemic body growth. IGF-1 is one of the most potent activators of the AKT signaling pathway, which stimulates cell proliferation and inhibits programmed cell death.

PODS are composed of a bioactive cargo protein within a polyhedrin crystal lattice, with polyhedrin as the main protein. The 25 µg SKU size indicates the amount of bioactive cargo, typically 0.5% to 5% of the total protein. For instance, a 25 µg SKU at a 2% cargo loading contains 1.25 mg of total protein. PODS enable sustained release of cargo proteins when proteases degrade the polyhedrin scaffold. Growth factors are not bioavailable until released, and their concentration in media depends on the amount added and the release rate. In 10% serum, peak levels are usually reached within 24-48 hours, with about 20% of the cargo becoming soluble.

PODS often require lower concentrations than conventional growth factors, as they maintain effective levels. Pre-incubating with serum or proteases enhances availability, while conventional factors can be added for immediate effects. Optimal amounts should be determined empirically.

PODS is a registered trademark of Cell Guidance Systems Ltd