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Merck

04-080

Anti-BAF250a/ARID1a Antibody, clone PSG3

clone PSG3, Upstate®, from mouse

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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
PSG3, monoclonal
Application:
IF, WB
Citations:
19
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biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

PSG3, monoclonal

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

immunofluorescence: suitable, western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

Gene Information

human ... ARID1A (8289)

General description

~270 kDa

Immunogen

GST fusion protein corresponding to 419 residues in the mid-portion of human BAF250a/ARID1a

Application

Research Sub Category
Chromatin Biology

Transcription Factors
Anti-BAF250a/ARID1a Antibody, clone PSG3 is an antibody against BAF250a/ARID1a for use in WB, ChIP, IC & IHC.
Research Category
Epigenetics & Nuclear Function

Biochem/physiol Actions

BAF250a/ARID1a

Physical form

100 μg of Protein A purified mouse monoclonal IgG in 100μl of 1X PBS, pH 7.0, 0.1% Azide.
Format: Purified
Protein A purified

Preparation Note

2 years at -20°C from date of shipment

Analysis Note

Routinely evaluated by immunoblot.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Clase de almacenamiento

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

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Coexistent ARID1A-PIK3CA mutations promote ovarian clear-cell tumorigenesis through pro-tumorigenic inflammatory cytokine signalling.
Chandler, RL; Damrauer, JS; Raab, JR; Schisler, JC; Wilkerson, MD; Didion, JP; Starmer et al.
Nature Communications null
Radhika Mathur et al.
Nature genetics, 49(2), 296-302 (2016-12-13)
Genes encoding subunits of SWI/SNF (BAF) chromatin-remodeling complexes are collectively mutated in ∼20% of all human cancers. Although ARID1A is the most frequent target of mutations, the mechanism by which its inactivation promotes tumorigenesis is unclear. Here we demonstrate that
Hiroko Inoue et al.
The Journal of biological chemistry, 277(44), 41674-41685 (2002-08-30)
The mammalian SWI/SNF-related complexes facilitate gene transcription by remodeling chromatin using the energy of ATP hydrolysis. The recruitment of these complexes to promoters remains poorly understood and may involve histone modifications or direct interactions with site-specific transcription factors or other
Xiaomei Wang et al.
The Biochemical journal, 383(Pt 2), 319-325 (2004-06-02)
p270 (ARID1A) is a member of the ARID family of DNA-binding proteins and a subunit of human SWI/SNF-related complexes, which use the energy generated by an integral ATPase subunit to remodel chromatin. ARID1B is an independent gene product with an
Guillermo Pablo Vicent et al.
The Journal of biological chemistry, 285(4), 2622-2631 (2009-11-27)
Steroid hormones induce transcription of their responsive genes by complex mechanisms including synergism between the hormone receptors and other transcription factors. On the mouse mammary tumor virus (MMTV) promoter progesterone induction is mediated by the reciprocal synergism between progesterone receptor

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