126575
Albumin, Bovine Serum, Fraction V, Fatty Acid-Free
Sinónimos:
Albumin, Bovine Serum, Fraction V, Fatty Acid-Free
Iniciar sesión para ver los precios por organización y contrato.
Seleccione un Tamaño
Acerca de este artículo
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarleServicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarledescription
Merck USA index - 14, 8468
assay
≥95% (cellulose acetate electrophoresis)
form
powder
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
≤0.02% fatty acids
≤3% ash
≤5% moisture
color
white to light yellow
light yellow to light brown
shipped in
ambient
storage temp.
2-8°C
Application
Albumin, Bovine Serum, Fraction V, Fatty Acid-Free has been used:
- as a component of: blocking buffer immunoelectron microscopy (IEM) for various cells
- mitochondrial isolation buffer (MIB) and mitochondria assay solution (MAS) to trap free fatty acids released during adipose tissue homogenization
- blocking buffer for wholemount staining of the esophageal epithelium (EE) in immunofluorescence
Biochem/physiol Actions
Bovine Serum Albumin (BSA), the most vital component of cell culture media, aids in embryonic stem cells (hESC) differentiation. It aids the transportation of drugs, hormones, and fatty acids. BSA is also used as a blocking agent in enzyme-linked immunosorbent assay (ELISA).
Disclaimer
Toxicity: Standard Handling (A)
General description
Albumin, Bovine Serum, (BSA) Fraction V, Fatty Acid-Free is useful for immunodiagnostic assays. It is designed for use in serological testing, radioimmunoassay, and hormone response studies. It is also applicable in binding and transport studies. BSA is prepared by cold ethanol extraction (Cohn method) with a pH: 6.8-7.4.
Bovine serum albumin (BSA) is a non-glycosylated globular, α-helical protein belonging to the serum albumin family. It consists of three domains with two sub-domains each and has 17-disulfide bonds.
Preparation Note
Following reconstitution, store in the refrigerator (4°C). 0.1% NaN₃ may be added as a preservative. Stock solutions are stable for up to 3 months at 4°C.
May take up to 24 h to fully dissolve.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
M Prats
European journal of biochemistry, 75(2), 619-625 (1977-05-16)
The L-(+)-Lactate:cytochrome c oxidoreductase or cytochrome b2 from the yeasts Saccharomyces cerevisiae and Hansenula anomala were partially hydrolysed in various concentrations of trypsin. Conditions were found which allowed the isolation from the Hansenula enzyme of a 140 000 +/- 10
Jacob M Serfass et al.
The Journal of biological chemistry, 292(24), 10097-10111 (2017-04-30)
Endocytosis, and the subsequent trafficking of endosomes, requires dynamic physical alterations in membrane shape that are mediated in part by endophilin proteins. The endophilin B family of proteins contains an N-terminal Bin/amphiphysin/Rvs (N-BAR) domain that induces membrane curvature to regulate
E Sandra Chocron et al.
Molecular endocrinology (Baltimore, Md.), 26(7), 1117-1128 (2012-05-10)
We previously demonstrated that the thyroid hormone, T(3), acutely stimulates mitochondrial metabolism in a thyroid hormone receptor (TR)-dependent manner. T(3) has also recently been shown to stimulate mitochondrial fatty acid oxidation (FAO). Here we report that TR-dependent stimulation of metabolism
Andrew W Ferree et al.
Autophagy, 9(11), 1887-1896 (2013-10-24)
To study mitochondrial protein age dynamics, we targeted a time-sensitive fluorescent protein, MitoTimer, to the mitochondrial matrix. Mitochondrial age was revealed by the integrated portions of young (green) and old (red) MitoTimer protein. Mitochondrial protein age was dependent on turnover
Soyeon Park et al.
Experimental & molecular medicine, 50(8), 1-14 (2018-08-05)
Increased fatty acid (FA) is often observed in highly proliferative tumors. FAs have been shown to modulate the secretion of proteins from tumor cells, contributing to tumor survival. However, the secreted factors affected by FA have not been systematically explored.
Contenido relacionado
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico