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Merck

393206

Anti-HNE Fluorophore Rabbit pAb

liquid, Calbiochem®

Sinónimos:

Anti-N α-Acetyllysine-4-hydroxy-2-nonenal Fluorophore, Anti-Nα-Acetyllysine-4-hydroxy-2-nonenal Fluorophore

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About This Item

NACRES:
NA.43
UNSPSC Code:
12352203

Nombre del producto

Anti-HNE Fluorophore Rabbit pAb, liquid, Calbiochem®

biological source

rabbit

antibody form

diluted serum

antibody product type

primary antibodies

clone

polyclonal

form

liquid

species reactivity (predicted by homology)

all

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

dilution

(ELISA (1:500-1:1000)
Immunoblotting (1:1000-1:5000)
Immunohistochemistry (1:100-1:500))

isotype

IgG

shipped in

wet ice

storage temp.

−70°C

target post-translational modification

unmodified

Quality Level

100

Immunogen

a 2:1 Nα-acetyllysine-HNE fluorophore, conjugated to KLH

Application

ELISA (1:500-1:1000)

Immunoblotting (1:1000-1:5000)

Immunohistochemistry (1:100-1:500)

Disclaimer

Toxicity: Standard Handling (A)

General description

Anti-HNE Fluorophore, rabbit polyclonal, recognizes the HNE-derived chromphohore of the 2:1 lysine-HNE-fluorophore. It is validated for use in ELISA, Western blotting, and immunohistochemistry.
Rabbit polyclonal antibody supplied as diluted serum. Recognizes the HNE-derived chromophore of the 2:1 lysine-HNE fluorophore.
Recognizes the HNE-derived chromphohore of the 2:1 lysine-HNE-fluorophore.

Other Notes

Due to the small epitope size, overnight incubation with the antibody greatly increases sensitivity by immunoblotting. Determined by competitive ELISA and immunoblotting to recognize the chromophore structure of fluorophore derived from the reaction of 4-HNE with primary amines. Does not recognize 1:1 cysteine-, 1:1 histidine-, or 1:1 lysine-HNE Michael Adducts. Variables associated with assay conditions will dictate the proper working dilution.
Tsai, L., et al. 1998. Proc. Natl. Acad. Sci. USA95, 7975.

Physical form

Serum diluted 1:1 with glycerol.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Clase de almacenamiento

10 - Combustible liquids

wgk

WGK 1

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