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Merck

AB3375

Anti-NFκB p65 Antibody, phospho-specific (Ser276)

serum, Chemicon®

Sinónimos:

Anti-Anti-CMCU, Anti-Anti-NFKB3, Anti-Anti-p65

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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
human
Application:
ELISA, WB
Citations:
4
Servicio técnico
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biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable, western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

phosphorylation (pSer276)

Gene Information

human ... NFKB1(4790)

Immunogen

Epitope: phospho-specific (Ser276)
Synthetic peptide from human NFkappaB p65, phosphoSer276.

Application

Anti-NFκB p65 Antibody, phospho-specific (Ser276) is a high quality Rabbit Polyclonal Antibody for the detection of NFκB p65 & has been validated in ELISA & WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Western blot: 1:1,000. A 65 kDa band is observed against human p65. HeLa cells of TNF inducible KBM-5 cells can be used as a positive control.

ELISA: 1:10,000-1:30,000

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

NFkappaB p65, phosphoSer276. By Western blot the antibody shows minimal reactivity with non-phosphorylated p65 and by ELISA is shows minimal reactivity with the non-phosphorylated form of the immunogen peptide.

Physical form

Rabbit serum. Liquid containing 0.1% sodium azide.

Preparation Note

Maintain at -20°C in undiluted aliquots up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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Clase de almacenamiento

10 - Combustible liquids

wgk

WGK 1



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John A Marwick et al.
Cell death & disease, 9(6), 665-665 (2018-06-06)
Apoptotic cells modulate the function of macrophages to control and resolve inflammation. Here, we show that neutrophils induce a rapid and sustained suppression of NF-κB signalling in the macrophage through a unique regulatory relationship which is independent of apoptosis. The
Terri Phua et al.
Scientific reports, 7, 44351-44351 (2017-03-14)
Many gastrointestinal diseases exhibit a protracted and aggravated inflammatory response that can lead to hypercytokinaemia, culminating in extensive tissue damage. Recently, angiopoietin-like 4 (ANGPTL4) has been implicated in many inflammation-associated diseases. However, how ANGPTL4 regulates colonic inflammation remains unclear. Herein
Monica Castellucci et al.
The Journal of allergy and clinical immunology, 136(3), 781-791 (2015-06-06)
IL-10 is well known for its ability to block the expression and production of numerous proinflammatory cytokines, in this manner preventing the development of excessive or chronic immune activation. IL-10-induced transcriptional repression of CXCL8 and TNFA genes consists of 2



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SKUGTIN
AB337504053252337796