Anti-MCAM/CD146 Antibody

KLH-conjugated linear peptide corresponding to a sequence from the third C2-type Ig-like domain of human MCAM/CD146.

~113 kDa observed. Target band size appears larger than the calculated molecular weights of 70.90 kDa (isoform 1) and 56.89 kDa (isoform 2) due to glycosylation.

Epitope: C2-type Ig-like domain 3.

Expected to react with both spliced isoforms of human MCAM/CD146 reported by UniProt (P43121).

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected MCAM/CD146 in 10 µg of HeLa cell lysate.

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