Anti-INF2 Antibody

INF2 (inverted formin 2) is a mouse formin that has been found to interact with actin through the C-terminus to the formin homology 2 domain. The WASP homology 2 motif activates another region that appropriates actin monomers. INF2 has a unique ability to speed up polymerization and depolymerization of actin filaments. Mutations in the N-terminal diaphanous inhibitory domain of INF2 have been proven to be a main cause of familial focal segmental glomerulosclerosis.

~155 kDa observed.
INF2 has been shown in WB to migrate as an ~155 kDa band (Madrid, R., et al. (2010). Dev Cell. 18(5):814-827).
Uniprot describes 3 isoforms produced by alternative splicing with MW′s @ ~135, ~134 & ~25 kDa

KLH-conjugated linear peptide corresponding to human INF2.

Anti-INF2 Antibody is an antibody against INF2 for use in WB, IH(P).

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot of this antibody detected INF2 in stomach cancer tissue.

Research Category
Cell Structure

Research Sub Category
Cytoskeletal Signaling

Other homologies: Mouse (68% sequence homology).

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl and 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
HeLa cell lysate

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.05 µg/mL of this antibody detected INF2 in 10 µg of HeLa cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.