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NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
DH01, monoclonal
Species reactivity:
human
Application:
—
Citations:
29
Servicio técnico
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Permítanos ayudarlebiological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
DH01, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
dilution
(Immunoblotting (1 µg/mL)
Immunoprecipitation (2 µg/mg protein lysate))
isotype
IgG1
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... CDH1(999)
General description
Anti-Cdh1 (Ab-2), mouse monoclonal, clone DH01, recognizes the ~55 kDa Cdh-1 protein in Rat-1 cells. It is validated for Western blotting and immunoprecipitation.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes the ~55 kDA Cdh1 protein.
Recognizes the ~55 kDa Cdh-1 protein in Rat-1 cells.
Immunogen
Human
recombinant human Cdh1 protein
Application

Immunoblotting (1 g/ml)
Immunoprecipitation (2 g/mg protein lysate)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 10 mM PBS, 0.2% BSA, pH 7.4.
Analysis Note
Negative Control
Normal mouse serum
Normal mouse serum
Positive Control
Junkat or LS174T cells
Junkat or LS174T cells
Other Notes
Does not cross-react with other WD repeat containing proteins, including Cdc20. Antibody should be titrated for optimal results in individual systems.
Kramer, E.R., et al. 2000 Mol. Biol. Cell11, 1555.
Petersen, B.O., et al. 2000 Genes Dev.14, 2330.
Pfleger, C.M., et al. 2001 Genes Dev.15, 1759.
Sorensen, C.S., et al. 2001 Mol. Cell Biol.21, 3692.
Gieffers, C., et al. 1999 Proc. Natl. Acad. Sci. USA96, 11317.
Visintin, R., et al. 1997 Science278, 460.
Petersen, B.O., et al. 2000 Genes Dev.14, 2330.
Pfleger, C.M., et al. 2001 Genes Dev.15, 1759.
Sorensen, C.S., et al. 2001 Mol. Cell Biol.21, 3692.
Gieffers, C., et al. 1999 Proc. Natl. Acad. Sci. USA96, 11317.
Visintin, R., et al. 1997 Science278, 460.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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APC/CCdh1 is a ubiquitin ligase with roles in numerous diverse processes, including control of cellular proliferation and multiple aspects of the DNA damage response. Precise regulation of APC/CCdh1 activity is central to efficient cell-cycle progression and cellular homeostasis. Here, we