Anti-Thy-1.1 Antibody, clone OX-7

Rat Thy-1

Anti-Thy-1.1 Antibody, clone OX-7 detects level of Thy-1.1 & has been published & validated for use in FC, IC, IH.

Flow Cytometry: 1:10-1:100. Use 10 μl per 10E6 thymocytes.

Immunohistochemistry on frozen sections.

Immunocytochemistry

Optimal working dilutions must be determined by end user.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

Directed against the Thy 1.1 antigenic determinant which is a monomorphic determinant within rat strains but polymorphic in the mouse (positive to AKR strain, negative to CVA and BALB/c). Thy-1 antigen is present on a variety of cell types including thymocytes, neuronal cells, T-lymphocytes (mouse), stem cells and immature B cells (rat) and connective tissue (Campbell, 1981). Thy-1 is a glycoprotein, 112 amino acids, which is homologous to immunoglobulin domains (Williams & Gagnon, 1982). MAB1406 can be coupled to sepharose 4-B and used to purify mouse Thy-1.1 and rat Thy-1 antigens (Campbell, 1981; Williams & Gagnon, 1982). MAB1406 can also be used to study the killing of Thy-1 tumor cells with antibody-toxin conjugates (Neville, 1982).

Format: Purified

Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.

Protein A purified

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Control
Thymocytes, T-lymphocytes

Tested

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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