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MABD76

Anti-Oct-4 Antibody, clone 9B7

Name: Anti-Oct-4 Antibody, clone 9B7
Brand: MM

clone 9B7, from mouse

Sinónimos:

POU domain, class 5, transcription factor 1, Octamer-binding protein 3, Oct-3, Octamer-binding protein 4, Oct-4, Octamer-binding transcription factor 3, OTF-3

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UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Conjugate:

unconjugated

Clone:

9B7, monoclonal

Application:

IF, IHC, WB

Citations:

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Propiedades

biological source

mouse

Quality Segment

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

9B7, monoclonal

species reactivity

mouse

species reactivity (predicted by homology)

human (immunogen homology)

technique(s)

immunofluorescence: suitable, immunohistochemistry: suitable, western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_002692

UniProt accession no.

Q01860

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... POU5F1(5460)

Descripción

General description

The POU5F1 gene expresses the POU-domain transcription factor, Octamer-4 (Oct-4), which is widely regarded as a hallmark of pluripotent stem cells. The relationship of Oct-4 to pluripotent stem cells is indicated by its tightly restricted expression to undifferentiated pluripotent stem cells. Upon differentiation to somatic lineages, the expression of Oct-4 disappears rapidly. Unlike the majority of pluripotent stem cell markers, the biological role of Oct-4 has been well characterized. Studies performed in mice point to the critical role of Oct-4 in the establishment and/or maintenance of pluripotent stem cells in an uncommitted state.

~39 kDa observed

Immunogen

Recombinant protein corresponding to human Oct-4.

Application

Anti-Oct-4 Antibody, clone 9B7 detects level of Oct-4 & has been published & validated for use in WB, IH & IF.

Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in human lung tissue.

Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in COS7 cells.

Research Category
Stem Cell Research

Research Sub Category
Transcription Factors

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse embryonic stem cell lysate

Evaluated by Western Blot in mouse embryonic stem cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Oct-4 in 10 µg of mouse embryonic stem cell lysate.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Clase de almacenamiento

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c