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MABT100

Anti-Girdin Antibody, CT, clone 10E6.1

clone 10E6.1, from mouse

Sinónimos:

Girdin, Akt phosphorylation enhancer, Coiled-coil domain-containing protein 88A, G alpha-interacting vesicle-associated protein, GIV, Girders of actin filament, Hook-related protein 1, HkRP1

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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
10E6.1, monoclonal
Application:
ICC, IHC, IP, WB
Citations:
3
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biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

10E6.1, monoclonal

species reactivity

human

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable, western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_001129069

UniProt accession no.

Q3V6T2

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CCDC88A(55704)

General description

Girdin (Ccdc88a; APE, GIV, or HkRP1) is a ubiquitous protein belonging to the CCDC88 family. Girdin is an actin-binding protein which interacts with actin via its C-terminal domain, and may play a crucial role in cell motility and cytoskeleton remodeling. These effects may be regulated by a reciprocal relationship between Girdin and Akt. Akt phosphorylates Girdin, and Girdin binds to Akt and enhances phosphorylation of Akt, thereby promoting phosphorylation of Akt’s downstream targets such as GSK-3β and FOXO1A. Girdin/Akt signaling may play an important role in cancer by facilitating the proliferation of cancer cells.
~216 kDa observed

Immunogen

Recombinant protein corresponding to human Girdin.

Application

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Girdin in epithelial cells of human prostate tissue.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Girdin in HeLa cells.

Immunoprecipitation Analysis: A representative lot immunoprecipitated Girdin in COS-7 cell lysate.
This Anti-Girdin, C-terminus Antibody, clone 10E6.1 is validated for use in Western Blotting, IHC(P), ICC, Immunoprecipitation for the detection of Girdin, C-terminus, clone 10E6.1.

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blot in human brain tissue lysate.

Wesetern Blot Analysis: A 1:5000 dilution of this antibody detected Girdin in 10 µg of human brain tissue lysate.

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Clase de almacenamiento

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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SKUGTIN
MABT10004053252643422