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Merck

OP44

Anti-APC (Ab-1) Mouse mAb (FE9)

liquid, clone FE9, Calbiochem®

Sinónimos:

Anti-Adenomatous Polyposis Coli

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NACRES:
NA.43
UNSPSC Code:
12352203
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Nombre del producto

Anti-APC (Ab-1) Mouse mAb (FE9), liquid, clone FE9, Calbiochem®

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

FE9, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

rat, human, mouse

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

dilution

(Immunoblotting (1 µg/mL))

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

Gene Information

human ... APC(324)

Analysis Note

Positive Control
HCT116 cells for p300, SW480 cells for truncated APC (p147)

Application

Immunoblotting (1 µg/ml, see comments)

Disclaimer

Toxicity: Standard Handling (A)

General description

Anti-APC (Ab-1), mouse monoclonal, clone FE9, recognizes full length APC (p300) in HCT116 cells and truncated APC (p147) in SW480 cells. It is validated for Western botting.
Protein G purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with SP40 cells. Recognizes the ~300 kDa APC protein as well as a variety of truncated forms.
Recognizes full length APC (p300) in HCT116 cells and truncated APC (p147) in SW480 cells.
  • Antibody Target Gene Symbol: APC
  • Target Synonym: AI047805, Apc7, AU020952, AW124434, BTPS2, DP2, DP2.5, DP3, Familial adenomatous polyposis, FAP, GS, Min, RATAPC
  • Entrez Gene Name: adenomatous polyposis coli
  • Hu Entrez ID: 324 (Related Antibodies: OP80, ST1150, OP62, OP47L)
  • Mu Entrez ID: 11789
  • Rat Entrez ID: 24205

Immunogen

a synthetic peptide corresponding to the N-terminal 35 amino acids of APC

Other Notes

Koetsier, P. A., et al. 1993. BioTechniques15, 258.
Smith, K. J., et al. 1993. Proc. Natl. Acad. Sci., USA90, 2846.
Su, L.-K., et al. 1993. Can. Res.53, 2728.
Boynton, R. F., et al. 1992. Proc. Natl. Acad. Sci. USA89, 3385.
D′Amico, D., et al. 1992. Cancer Res.52, 1996.
Fearon, E. R., and Jones, P. A., 1992. FASEB J.6, 2783.
Miyoshi, Y., et al. 1992. Proc. Natl. Acad. Sci. USA89, 4452.
Powell, S. M., et al. 1992. Nature359, 235.
Groden, J., et al. 1991. Cell66, 589.
Kinzler, K. W., et al. 1991. Science253, 661.
Nishisho, I., et al. 1991. Science253, 665.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, pH 7.5, 0.2% gelatin.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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