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Merck

32211

Cloroformo

puriss. p.a., reag. ISO, reag. Ph. Eur., 99.0-99.4% (GC)

Sinónimos:

Triclorometano, Tricloruro de metilidino

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Fórmula empírica (notación de Hill):
CHCl3
Número CAS:
Peso molecular:
119.38
UNSPSC Code:
12191502
NACRES:
NA.04
PubChem Substance ID:
EC Number:
200-663-8
Beilstein/REAXYS Number:
1731042
MDL number:
Assay:
99.0-99.4% (GC)
Technique(s):
RNA extraction: suitable
Bp:
60.5-61.5 °C (lit.)
Vapor pressure:
160 mmHg ( 20 °C)
Servicio técnico
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Quality Level

agency

USP/NF, reag. ISO, reag. Ph. Eur.

vapor density

4.1 (vs air)

vapor pressure

160 mmHg ( 20 °C)

grade

puriss. p.a.

assay

99.0-99.4% (GC)

form

liquid

contains

~1% ethanol as stabilizer

technique(s)

RNA extraction: suitable

impurities

≤0.00001% free chlorine (Cl), ≤0.00005% free acid (as HCl), ≤0.0005% non-volatile matter, ≤0.005% aldehydes and ketones (as CH3COCH3), ≤0.005% carbonyl compounds (as CO), ≤0.01% tetrachloroethene (GC), ≤0.01% tetrachloromethane (GC), ≤0.01% trichloroethene (GC), ≤0.01% water (Karl Fischer), ≤0.03% dichloromethane (GC), 0.6-1.0% ethanol (GC)

refractive index

n20/D 1.445 (lit.)

bp

60.5-61.5 °C (lit.)

mp

−63 °C (lit.)

density

1.476-1.483 g/mL at 20 °C, 1.492 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤1 mg/kg

SMILES string

ClC(Cl)Cl

cation traces

Al: ≤0.5 mg/kg, B: ≤0.02 mg/kg, Ba: ≤0.1 mg/kg, Ca: ≤0.5 mg/kg, Cd: ≤0.05 mg/kg, Co: ≤0.02 mg/kg, Cr: ≤0.02 mg/kg, Cu: ≤0.02 mg/kg, Fe: ≤0.1 mg/kg, Mg: ≤0.1 mg/kg, Mn: ≤0.02 mg/kg, Ni: ≤0.02 mg/kg, Pb: ≤0.05 mg/kg, Sn: ≤0.1 mg/kg, Zn: ≤0.1 mg/kg

suitability

complies for appearance, complies for reaction against H2SO4, complies for suitability of determ. w. dithizone

InChI

1S/CHCl3/c2-1(3)4/h1H

InChI key

HEDRZPFGACZZDS-UHFFFAOYSA-N

General description

Chloroform, a halogenated hydrocarbon, is widely employed as a solvent. Its photochemical degradation has been investigated in the presence of TiO2 aqueous suspensions (wavelength range of 310-380nm).

Application

Chloroform may be used as a solvent to enhance the enantioselectivity of secondary alcohols formed by the borohydride reduction of carbonyl compounds in the presence of optically active ketoiminatocobalt complexes.
Chloroform may be employed as a solvent for the following studies:
  • Extraction of RNA from plant cells.
  • Preparation of dioleoylphosphatidylcholine (DOPC) solution.
  • Dissolution of 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC).
It may be employed in a rapid quantitative method for the release of periplasmic proteins from bacterial strains.

Other Notes

For information on chloroform miscibility, please visit the following link:
Chloroform Miscibility/Immiscibility Table
The article number 32211-4X2.5L will be discontinued. Please order the single bottle 32211-2.5L which is physically identical with the same exact specifications.
The article number 32211-4X2.5L-M will be discontinued. Please order the single bottle 32211-2.5L-M which is physically identical with the same exact specifications.
The article number 32211-6X1L will be discontinued. Please order the single bottle 32211-1L which is physically identical with the same exact specifications.
The article number 32211-6X1L-M will be discontinued. Please order the single bottle 32211-1L-M which is physically identical with the same exact specifications.


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pictograms

Skull and crossbonesHealth hazard

signalword

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 3

target_organs

Central nervous system, Liver,Kidney

Clase de almacenamiento

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk

WGK 3

flash_point_f

does not flash

flash_point_c

does not flash



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Chloroform is not solvent but activator for cobalt complex catalyst of enantioselective borohydride reduction.
Kokura, Ai, et al.
Chemistry Letters (Jpn), 36(1), 26-27 (2006)
Jameel A Feshitan et al.
Journal of colloid and interface science, 329(2), 316-324 (2008-10-28)
Microbubbles used as contrast agents for ultrasound imaging, vectors for targeted drug delivery and vehicles for metabolic gas transport require better size control for improved performance. Mechanical agitation is the only method currently available to produce microbubbles in sufficient yields
Z V Leonenko et al.
Biochimica et biophysica acta, 1509(1-2), 131-147 (2000-12-19)
We have used magnetic alternating current mode atomic force microscopy (MAC-AFM) to investigate the formation of supported phospholipid bilayers (SPB) by the method of vesicle fusion. The systems studied were dioleoylphosphatidylcholine (DOPC) on mica and mica modified with 3-aminopropyl-triethoxy-silane (APTES)