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Merck

A0480

Anti-Actin (plant) antibody, Mouse monoclonal

clone 10-B3 (MAbGPa), purified from hybridoma cell culture

Sinónimos:

Monoclonal Anti-Actin (plant) antibody produced in mouse

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UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD00145889
Conjugate:
unconjugated
Clone:
10-B3 (MAbGPa), monoclonal
Application:
immunohistochemistry
indirect ELISA
western blot
Species reactivity:
plant
Citations:
145
Technique(s):
immunohistochemistry: suitable
indirect ELISA: suitable
western blot: 1-2 μg/mL using corn leaves extracts

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Nombre del producto

Anti-Actin (plant) antibody, Mouse monoclonal, clone 10-B3 (MAbGPa), purified from hybridoma cell culture

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

10-B3 (MAbGPa), monoclonal

mol wt

antigen ~45 kDa

species reactivity

plant

packaging

antibody small pack of 25 μL

technique(s)

immunohistochemistry: suitable
indirect ELISA: suitable
western blot: 1-2 μg/mL using corn leaves extracts

isotype

IgG2b

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

Arabidopsis thaliana ... ACT8(841347)

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Application

Monoclonal Anti-Actin (plant) antibody produced in mouse has been used in:
  • two-layer phos-tag polyacrylamide gel electrophoresis (PAGE)
  • immunohistochemistry and enzyme-linked immunosorbent assay (ELISA).
  • immunoblotting

Biochem/physiol Actions

In Arabidopsis ACT7 isovariant is essential for normal phytohormone response to hormones in different organs. Mutation in the actin (ACT)1 gene is associated with the development of dwarfism in plants in addition to delayed flowering, highly reduced organ size and varied branching pattern of the leaf trichomes and inflorescence stem. In plants, there is a developmental switch in the modulation of actin isovariants throughout cell differentiation and maturation phase.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Arabidopsis has a complex actin gene family that encodes six protein subclasses. One of the classes is represented by the proteins ACT2 and ACT8, which are similar proteins, but have different flanking sequences, introns and silent nucleotide positions in codons. ACT8 is expressed in root, stems, leaves, flowers, pollen and siliques.
Monoclonal Anti-Actin (plant) antibody recognizes actin in several plant species including Dictyostelium. In Arabidopsis, the antibody recognizes all eight actins isoforms (ACT1, 2, 3, 4, 7, 8, 11 and 12).
Monoclonal Anti-Actin (plant) (mouse IgG2b isotype) is derived from the hybridoma 10-B3 (MabGPa) produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice immunized with ACT8 protein. In Arabidopsis, actin is divided into two major phylogenetic classes namely reproductive and vegetative class.

Immunogen

ACT8 protein.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

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Clase de almacenamiento

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000521924
0000521924
0000478535
0000478535
0000407500

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Functional Nonequivalency of Actin Isovariants in
Kandasamy, et al.
Molecular Biology of the Cell, 13(1), 251-251 (2002)
Andrés Ritter et al.
Nature communications, 8, 15235-15235 (2017-05-12)
Most living organisms developed systems to efficiently time environmental changes. The plant-clock acts in coordination with external signals to generate output responses determining seasonal growth and flowering time. Here, we show that two Arabidopsis thaliana transcription factors, FAR1 RELATED SEQUENCE
Phosphorylation of the light-harvesting complex II isoform Lhcb2 is central to state transitions
Longoni P, et al.
Plant Physiology, 169(4), 2874-2883 (2015)
An Endogenous Carbon-Sensing Pathway Triggers Increased Auxin Flux and Hypocotyl Elongation
Lilley JL, et al.
Plant Physiology, 160(4), 2261-2270 (2012)
Hong-Xian Hsing et al.
Botanical studies, 57(1), 30-30 (2017-06-10)
Phalaenopsis orchid (Phal. orchid) is visually attractive and it is important economic floriculture species. Phal. orchids have many unique biological features. However, investigation of these features and validation on their biological functions are limited due to the lack of an
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