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Merck

A4058

Acrylamide solution

40%, suitable for electrophoresis, sterile-filtered

Sinónimos:

2-Propenamide, 40% Acrylamido solution, Acrylic amide, Akrylamid, Polyacrylamide solution

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About This Item

Fórmula lineal:
CH2=CHCONH2
Número CAS:
79-06-1
Peso molecular:
71.08
NACRES:
NB.22
PubChem Substance ID:
24890742
eCl@ss:
39031205
UNSPSC Code:
41105319
MDL number:
MFCD00008032
Beilstein/REAXYS Number:
6185892

Nombre del producto

Acrylamide solution, 40%, suitable for electrophoresis, sterile-filtered

InChI key

HRPVXLWXLXDGHG-UHFFFAOYSA-N

InChI

1S/C3H5NO/c1-2-3(4)5/h2H,1H2,(H2,4,5)

SMILES string

NC(=O)C=C

sterility

sterile-filtered

form

liquid

concentration

40%

technique(s)

electrophoresis: suitable

storage temp.

2-8°C

Quality Level

200

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Application

Acrylamide solution is suitable for use in electrophoresis. It may be used in the preparation of gel buffer for the determination of molecular weight of polypeptide chains by polyacrylamide gel electrophoresis. It may be used in the electrochemical polymerization of pyrrole. It may be used to prepare the pH 7.6 gels, required for the determination of molecular weights of histone by sodium dodecyl sulfate gel electrophoresis.

General description

Acrylamide has been classified as probably carcinogenic in humans. It is formed in fried and oven-cooked foods due to Maillard reaction between amino acids and reducing sugars.

Preparation Note

Prepared from electrophoresis grade acrylamide in ultra-pure water.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

target_organs

Peripheral nervous system

Clase de almacenamiento

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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Certificados de análisis (COA)

Lot/Batch Number
0000480675
0000480675
0000452027
0000452027
0000439370

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Electrochemical polymerization of pyrrole in acrylamide solution.
Sarac AS, et al.
Synthetic Metals, 98(3), 177-182 (1999)
Donald S Mottram et al.
Nature, 419(6906), 448-449 (2002-10-09)
Reports of the presence of acrylamide in a range of fried and oven-cooked foods have caused worldwide concern because this compound has been classified as probably carcinogenic in humans. Here we show how acrylamide can be generated from food components
The molecular weights of vertebrate histones exploiting a modified sodium dodecyl sulfate electrophoretic method.
S Panyim et al.
The Journal of biological chemistry, 246(24), 7557-7560 (1971-12-25)
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
K Weber et al.
The Journal of biological chemistry, 244(16), 4406-4412 (1969-08-25)
Arianna Rath et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(39), 15668-15673 (2013-09-11)
SDS/PAGE is universally used in biochemistry, cell biology, and immunology to resolve minute protein amounts readily from tissue and cell extracts. Although molecular weights of water-soluble proteins are reliably determined from their SDS/PAGE mobility, most helical membrane proteins, which comprise
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