Anti-Sheep IgG (whole molecule)–Alkaline Phosphatase antibody produced in donkey

Affinity isolated antibody is obtained from anti-sheep IgG antiserum by immunospecific purification which removes essentially all donkey serum proteins, including immunoglobulins, which do not specifically bind to sheep IgG. Anti-Sheep IgG is conjugated to alkaline phosphatase by protein cross-linking with 0.2% glutaraldehyde. Specificity of the antiserum is determined by immunoelectrophoresis (IEP), prior to conjugation. By IEP, the antiserum reacts specifically with normal sheep serum and sheep IgG. Identity and purity of the antibody is established by immunoelectrophoresis, prior to conjugation. Electrophoresis of the product followed by diffusion against anti-horse IgG and anti-donkey whole serum results in single arcs of precipitation.

IgGs are glycoprotein antibodies that modulate several immune responses. Sheep IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-sheep IgGs are useful tools for the analysis of target proteins. Anti-Sheep IgG (whole molecule)-Alkaline Phosphatase antibody binds to all sheep immunoglobulins.

Purified sheep IgG

Anti-Sheep IgG (whole molecule)-Alkaline Phosphatase antibody is suitable for use in ELISA .

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Solid-Phase Decapeptide Assays were performed using alkaline phosphatase conjugated donkey anti-sheep IgG at 1:1000 for 2-3 hours at room temperature.

The anibody may be used in applications such as immunoblotting, dot blot and direct ELISA at a dilution of 1:30,000

normal sheep serum and sheep IgG

Solution in 0.05 M Tris, pH 8.0, containing 1 mM MgCl₂, 50% glycerol, 1% bovine serum albumin, and 15 mM sodium azide.

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