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A6559

Adenosine 5′-triphosphate disodium salt solution

Name: Adenosine 5&#8242;-triphosphate disodium salt solution
Brand: SIGMA

≥95% (HPLC), liquid

Sinónimos:

ATP

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Número CAS:

987-65-5

UNSPSC Code:

41106305

eCl@ss:

32160414

PubChem Substance ID:

24891106

NACRES:

NA.52

MDL number:

MFCD00080339

Assay:

≥95%

Biological source:

yeast

Form:

liquid

Storage temp.:

−20°C

Servicio técnico

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Propiedades

Nombre del producto

Adenosine 5′-triphosphate disodium salt solution, Crystalline ATP, HPLC purified, aqueous solution for RNA transcription

biological source

yeast

Quality Level

300

assay

≥95%

form

liquid

concentration

100 mM in H₂O (adjusted to pH 7 with Trizma^®
base)

foreign activity

DNase, RNase, none detected

shipped in

dry ice

storage temp.

−20°C

SMILES string

[Na+].[Na+].Nc1ncnc2n(cnc12)[C@@H]3O[C@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)[C@@H](O)[C@H]3O

InChI

1S/C10H16N5O13P3.2Na/c11-8-5-9(13-2-12-8)15(3-14-5)10-7(17)6(16)4(26-10)1-25-30(21,22)28-31(23,24)27-29(18,19)20;;/h2-4,6-7,10,16-17H,1H2,(H,21,22)(H,23,24)(H2,11,12,13)(H2,18,19,20);;/q;2*+1/p-2/t4-,6-,7-,10-;;/m1../s1

InChI key

TTWYZDPBDWHJOR-IDIVVRGQSA-L

Descripción

General description

Adenosine 5′-triphosphate disodium salt solution is suitable for use in molecular biology applications such as kinase reactions or DNA-dependent RNA polymerase transcription.

Application

Adenosine 5′-triphosphate disodium salt solution has been used:

as a standard to evaluate the extracellular ATP release from marginal cells of stria vascular
as a component of the internal pipette solution to establish a recording electrode to study neurite stretching
in in vitro VPS34 (catalytic subunit of the phosphatidylinositol 3-kinase (PtdIns3K) complex) activity assay
as a component of lysis and resealing buffers for ATP studies
in spectrophotometric measurement of H⁺ transport in fluorescein isothiocyanate (FITC)-dextran loaded A549 (adenocarcinomic human alveolar basal epithelial cells) and HeLa cells
to induce pyroptosis in lipopolysaccharide treated fibroblast-like synoviocytes (FLSs)

Biochem/physiol Actions

Adenosine 5′-triphosphate (ATP) is an important energy currency in all living organisms. It possesses high phosphate transfer potential. ATP is involved in several biological processes such as membrane transport, muscle contraction and synthesis, and degradation of biological molecules. It plays a role as an intracellular and extracellular signaling molecule in certain cellular processes such as cell motility, organ development, neurotransmission, and insulin secretion.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Clase de almacenamiento

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Contenido relacionado

Product Information Sheet - A6559

Product Information Sheet

Cooper GM

The Cell: A Molecular Approach (2000)

Identification of small molecule inhibitors of phosphatidylinositol 3-kinase and autophagy.

Farkas T

The Journal of Biological Chemistry, 286(45), 38904-38912 (2011)

DRAM1 regulates autophagy flux through lysosomes.

Zhang XD

PLoS ONE, 8(5), e63245-e63245 (2013)

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