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A9357

Anti-alpha-Actin Antibody, Cardiac Muscle (ACTC1) Antibody

mouse monoclonal, AC1-20.4.2

Sinónimos:

Anti-Actin alpha cardiac muscle 1, Anti-ACTC, Anti-ACTC1, Anti-CMD1R, Anti-CMH11

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UNSPSC Code:
12352203
NACRES:
NA.41
Conjugate:
unconjugated
Clone:
AC1-20.4.2, monoclonal
Application:
ELISA (i), ICC, IHC (p), WB
Citations:
27
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Nombre del producto

Anti-Actin, Cardiac antibody, Mouse monoclonal, clone AC1-20.4.2, purified from hybridoma cell culture

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

AC1-20.4.2, monoclonal

form

buffered aqueous solution

mol wt

antigen ~42 kDa

species reactivity

rat, human, bovine, chicken, mouse

concentration

~1.0 mg/mL

technique(s)

immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 10-20 μg/mL using digested rat heart tissue, indirect ELISA: suitable, western blot: suitable

isotype

IgG1

UniProt accession no.

P68032

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ACTC1(70)
mouse ... Actc1(11464)
rat ... Actc1(29275)

General description

Actins are cytoskeletal proteins that are widely expressed in eukaryotes. These proteins primarily regulate various cellular functions such as muscle contraction, mRNA synthesis and apoptosis among many others. ACTC1 is the predominant actin isoform form in adult cardiac tissues and alterations in this isoform have been associated with hypertrophic cardiomyopathy and congenital heart disease . Monoclonal Anti-Actin, Cardiac antibody is specific for human, bovine, chicken, rat, and mouse cardiac α actin.

Immunogen

synthetic NH2-terminal decapeptide of cardiac isoform of actin.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)
Monoclonal Anti-Actin, Cardiac antibody is suitable for use in immunocytochemistry, immunoblot (approx. 42 kDa), indirect ELISA, and western blot. The antibody may also be used in immunohistochemistry (using formalin-fixed, paraffin-embedded sections, or using a conc. of 10-20 μg/mL in digested rat heart tissue).
Protein samples isolated from cardiac sections or cardiac myocytes were subjected to western blot using monoclonal mouse anti-actin antibody as the primary at a dilution of 1:2000.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

wgk

WGK 1

Clase de almacenamiento

12 - Non Combustible Liquids

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Certificados de análisis (COA)

Lot/Batch Number
0000426486
0000426486
0000397426
0000397426
0000251263

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Contenido relacionado

Data Sheet - A9357

Instructions

W W Franke et al.
Differentiation; research in biological diversity, 60(4), 245-250 (1996-07-01)
We describe three murine monoclonal antibodies (mAbs) raised against a synthetic decapeptide representing the aminoterminal sequence of the cardiac/ fetal isoform of sarcomeric alpha-actin. When used for immunoblotting or histological immunolocalization, these mAbs distinguish cardiac/fetal alpha-actin from skeletal muscle alpha-actin
Pratik A Lalit et al.
Nature protocols, 12(5), 1029-1054 (2017-04-21)
Here we describe a protocol to generate expandable and multipotent induced cardiac progenitor cells (iCPCs) from mouse adult fibroblasts using forced expression of Mesp1, Tbx5, Gata4, Nkx2.5 and Baf60c (MTGNB) along with activation of Wnt and JAK/STAT signaling. This method
Hong-Kun Jiang et al.
Circulation journal : official journal of the Japanese Circulation Society, 74(11), 2410-2418 (2010-10-22)
The Cardiac α actin 1 gene (ACTC1) has been related to familial atrial septal defects. This study was set to explore a potential role of this gene in the formation of sporadic congenital heart disease (CHD). Assessment of cardiac tissue
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