A9728
Monoclonal Anti-ATP Synthase β antibody produced in mouse
1 mg/mL, clone 4.3E8.D10, purified immunoglobulin, buffered aqueous solution
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Conjugate:
unconjugated
Clone:
4.3E8.D10, monoclonal
Application:
IF, IP, WB
Citations:
6
Servicio técnico
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Permítanos ayudarlebiological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
4.3E8.D10, monoclonal
form
buffered aqueous solution
mol wt
antigen 50 kDa
species reactivity
human, mouse, rat
concentration
1 mg/mL
technique(s)
immunoprecipitation (IP): suitable, indirect immunofluorescence: suitable, western blot: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... ATP5B(506)
mouse ... Atp5b(11947)
rat ... Atp5b(171374)
General description
ATP synthase is a highly conserved transmembrane protein that can catalyse the reversible synthesis of ATP from ADP and phosphate.
Detects the β subunit of ATP synthase and can be used as a mitochondrial marker.
Immunogen
intact rat mitochondria.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Western Blotting (1 paper)
Immunofluorescence (1 paper)
Western Blotting (1 paper)
Mouse Monoclonal Anti-ATP Synthase beta antibody can be used for western blot, indirect immunofluorescence and immunoprecipitation assays.
Pancreatic islet cells isolated from wither Wistar ratos or nondiabetic organ donors were fixed in 4% paraformaldhyde and permeabilized with 0.3% Triton-X for immunocytochemistry using monoclonal mouse anti- beta ATP synthase at a 1:1000 dilution.
Physical form
Solution in phosphate buffered saline containing 1.0 mg/mL bovine serum albumin and 0.05% sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Clase de almacenamiento
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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J Alfonso Leyva et al.
Molecular membrane biology, 20(1), 27-33 (2003-05-15)
To couple the energy present in the electrochemical proton gradient, established across the mitochondrial membrane by the respiratory chain, to the formation of ATP from ADP and Pi, ATP-synthase goes through a sequence of coordinated conformational changes of its major
Esteban N Gurzov et al.
The Journal of biological chemistry, 285(26), 19910-19920 (2010-04-28)
Type 1 diabetes is an autoimmune disorder characterized by chronic inflammation and pancreatic beta-cell loss. Here, we demonstrate that the proinflammatory cytokine interleukin-1beta, combined with interferon-gamma, induces the expression of the Bcl-2 homology 3 (BH3)-only activator PUMA (p53 up-regulated modulator
Sarah Engeham et al.
Journal of nutrition and metabolism, 2012, 989037-989037 (2012-04-27)
Maternal protein restriction in rat pregnancy is associated with impaired renal development and age-related loss of renal function in the resulting offspring. Pregnant rats were fed either control or low-protein (LP) diets, and kidneys from their male offspring were collected