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Merck

B7639

(+)-Biotin hydrazide

≥97% (TLC), powder

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Fórmula empírica (notación de Hill):
C10H18N4O2S
Número CAS:
Peso molecular:
258.34
UNSPSC Code:
12352203
NACRES:
NA.46
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
28347
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Nombre del producto

(+)-Biotin hydrazide, ≥97% (TLC), powder

InChI

1S/C10H18N4O2S/c11-14-8(15)4-2-1-3-7-9-6(5-17-7)12-10(16)13-9/h6-7,9H,1-5,11H2,(H,14,15)(H2,12,13,16)/t6-,7-,9-/m0/s1

SMILES string

[H][C@]12CS[C@@H](CCCCC(=O)NN)[C@@]1([H])NC(=O)N2

InChI key

KOZWHQPRAOJMBN-ZKWXMUAHSA-N

assay

≥97% (TLC)

form

powder

solubility

DMSO: ≤20 mg/mL

storage temp.

2-8°C

Quality Level

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Application

(+)-Biotin hydrazide has been used:
  • the modification of alginate
  • for the labelling of mitochondrial proteins from non-muscle tissues
  • as a component of glycoprotein staining solution
  • in periodic acid-biotin-hydrazide (PABH) assay for mucins
  • for labeling surface functional groups, biologically active molecules such as antibodies, lectins, sugars, nucleic acids or molecules with free carboxylic or keto groups.
  • for coupling to glycoproteins through the carbohydrate by hydrazone formation

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Biotin hydrazide is a biotinylation reagent used to biotinylate glycoproteins with their sugar moieties. Biotin hydrazide can be used to prepare biotin-conjugated alginate. It can also be used for covalent attachment to PAAc via carbodi-imide cross linking.

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Boris Polyak et al.
Biomacromolecules, 5(2), 389-396 (2004-03-09)
Biotin was covalently coupled with alginate in an aqueous-phase reaction by means of carbodiimide-mediated activation chemistry to provide a biotin-alginate conjugate for subsequent use in biosensor applications. The synthetic procedure was optimized with respect to pH of the reaction medium
Studying mucin secretion from human bronchial epithelial cell primary cultures
Mucins, 259-277 (2012)
Ajay M Shah et al.
Analytical chemistry, 84(8), 3682-3688 (2012-03-15)
Microfluidic systems for affinity-based cell isolation have emerged as a promising approach for the isolation of specific cells from complex matrices (i.e., circulating tumor cells in whole blood). However, these technologies remain limited by the lack of reliable methods for
Enhanced capture and release of circulating tumor cells using hollow glass microspheres with a nanostructured surface
Dong Z, et al.
Nanoscale, 10(35), 16795-16804 (2018)
E P Diamandis et al.
Clinical chemistry, 37(5), 625-636 (1991-05-01)
The biotin-(strept)avidin system has been used for many years in a variety of different applications. Here we present a general overview of the system, describe its components and advantages, and show how the system is used in various applications, with

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