B9285
5-Bromo-2′-desoxiuridina
≥99%, synthetic (organic), powder
Sinónimos:
5- BrdU, 5-Bromo-1-(2-desoxi-β-D-ribofuranosil)uracilo, BUdR, Desoxirribósido de 5-bromouracilo
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About This Item
Nombre del producto
5-Bromo-2′-desoxiuridina, BioUltra, ≥99%
InChI key
WOVKYSAHUYNSMH-RRKCRQDMSA-N
InChI
1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1
SMILES string
OC[C@H]1O[C@H](C[C@@H]1O)N2C=C(Br)C(=O)NC2=O
biological source
synthetic (organic)
product line
BioUltra
assay
≥99%
form
powder
impurities
≤0.005% Phosphorus (P)
≤0.1% Insoluble matter
ign. residue
≤0.1%
mp
191-194 °C (dec.) (lit.)
solubility
NH4OH: 0.1 M at 20 °C, clear, colorless
cation traces
Al: ≤0.0005%
Ca: ≤0.01%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Mg: ≤0.0005%
NH4+: ≤0.05%
Na: ≤0.05%
Pb: ≤0.001%
Zn: ≤0.0005%
storage temp.
−20°C
Quality Level
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Categorías relacionadas
Biochem/physiol Actions
Application
- to label proliferating cells in pregnant mice in cell proliferation assay
- to study the time course of cell proliferation at various times following ischemia, to confirm re-entry of MKI67+ cells in cell cycle to germ cells
- in 5′-Bromo-2′Deoxyuridine (BrdUrd) staining of fibroblasts
General description
signalword
Danger
hcodes
Hazard Classifications
Muta. 1B - Repr. 2
Clase de almacenamiento
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 2
ppe
Eyeshields, Gloves, type N95 (US)
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Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Can Product B9285, 5-Bromo-2´-deoxyuridine, be injected into animals?
Sigma has not injected animals with solutions of this compound, so we do not have a stepwise procedure for you. However, this application has been reported in the literature. Please see the cited references below, which you may find useful. Several references cite the use of Product No. B9285 in their protocols.1. Yin, X-X, et al., Expression of active caspase-3 in mitotic and postmitotic cells of the rat forebrain. J. Comp. Neurol., 433(1), 4-22 (2001). Injected into mice at 50 mg/kg.2. Dackor, R.T., et al., Hydrops Fetalis, Cardiovascular Defects, and Embryonic Lethality in Mice Lacking the Calcitonin Receptor-Like Receptor Gene. Mol. Cell. Biol., 26(7), 2511-2518 (2006). Injected into mice at 100 mg/kg.3. Farris, S.M., et al., Larval and Pupal Development of the Mushroom Bodies in the Honey Bee, Apis mellifera. J. Comp. Neurol., 414, 97-113 (1999). A 25 mg/mL solution was injected into bee larvae, prepupae, and pupae.4. Gaiano, N., et al., Radial Glial Identity Is Promoted by Notch1 Signaling in the Murine Forebrain. Neuron, 26(2), 395-404 (2000). A 20 mg/mL solution was injected into mice.
For how long is Product B9285, 5-Bromo-2´-deoxyuridine, stable in solution?
5-Bromo-2´-deoxyuridine is stable in solution for 6 months when stored -20°C.
In what solvents is Product B9285, 5-Bromo-2´-deoxyuridine, soluble?
Solubility information for 5-bromo-2´-deoxyuridine can be found on the product information sheet (under Documents, above).
What is the shelf life of Product No. B9285 (5-bromo-2´-deoxyuridine)?
Sigma-Aldrich does not assign an expiration date to this product; it is guaranteed for one year from the date of shipment.
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