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D5905

3,3′-Diaminobenzidine tetrahydrochloride

Name: 3,3&#8242;-Diaminobenzidine tetrahydrochloride
Brand: SIGMA

peroxidase substrate, chromogenic, tablet

Sinónimos:

3,3′,4,4′-Biphenyltetramine tetrahydrochloride, 3,3′,4,4′-Tetraaminobiphenyl tetrahydrochloride, DAB

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Fórmula lineal:

(NH₂)₂C₆H₃C₆H₃(NH₂)₂ · 4HCl

Peso molecular:

360.11

NACRES:

NA.83

PubChem Substance ID:

24893981

UNSPSC Code:

12352204

EC Number:

231-018-9

MDL number:

MFCD00012969

Beilstein/REAXYS Number:

1212988

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Propiedades

Nombre del producto

3,3′-Diaminobenzidine tetrahydrochloride, tablet, 10 mg substrate per tablet

Quality Level

200

form

tablet

solubility

water: 1 tablet/15 mL (may contain small insoluble particles)

storage temp.

−20°C

SMILES string

Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4.4ClH/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;/h1-6H,13-16H2;4*1H

InChI key

KJDSORYAHBAGPP-UHFFFAOYSA-N

Descripción

Application

Reagent for spectrophotometric determination of selenium.

3,3′-Diaminobenzidine tetrahydrochloride has been used for immunohistochemistry.

Biochem/physiol Actions

Peroxidase substrate suitable for use in immunoblotting and immunohistological staining procedures. This substrate produces an insoluble end product that is brown in color and can be observed visually.

3,3′-Diaminobenzidine tetrahydrochloride is a chromogenic substrate, which is commonly used in immunoperoxidase tests.

Preparation Note

Dissolve 1 tablet in 15 ml of Tris-buffered saline, pH 7.6. Add 12 μl of fresh 30% hydrogen peroxide. DAB and hydrogen peroxide concentration may be adjusted to suit individual applications.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

pictograms

GHS08,GHS07

signalword

Danger

hcodes

H302,H319,H341,H350

pcodes

P202 - P264 - P280 - P301 + P312 - P305 + P351 + P338 - P308 + P313

Hazard Classifications

Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2

Clase de almacenamiento

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

ppe

Eyeshields, Gloves, type N95 (US)

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