D8168
Anti-Dystrophin Antibody
mouse monoclonal, MANDYS8
Sinónimos:
Dystrophin Antibody, Dystrophin Antibody - Monoclonal Anti-Dystrophin antibody produced in mouse
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Acerca de este artículo
Conjugate:
unconjugated
Clone:
MANDYS8, monoclonal
Application:
ARR, ELISA (i), IF, WB
Citations:
71
Servicio técnico
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Permítanos ayudarleNombre del producto
Monoclonal Anti-Dystrophin antibody produced in mouse, clone MANDYS8, ascites fluid
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
MANDYS8, monoclonal
species reactivity
chicken, rat, human, pig, rabbit, mouse
technique(s)
indirect ELISA: suitable, indirect immunofluorescence: 1:400 using frozen human or animal muscle tissue sections., microarray: suitable, western blot: suitable
isotype
IgG2b
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... DMD(1756)
mouse ... Dmd(13405)
rat ... Dmd(24907)
General description
Monoclonal Anti-Dystrophin (mouse IgG2b isotype) is derived from the MANDYS8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Dystrophin is a structural protein on the inner face of the membrane, consisting of a 25-repeat, rod-like, triple-helical domain separating an N-terminal actin binding domain from two C-terminal domains, one of which is rich in cysteine.
The rod domain of the human dystrophin molecule is present in normal muscle tissue and in nearly all Becker muscular dystrophies. It is absent in the cases of Duchenne muscular dystrophies and in the dystrophic mouse (mdx).
Immunogen
recombinant human dystrophin fragment.
Application
Monoclonal Anti-Dystrophin antibody produced in mouse has been used in
- immunohistochemistry
- immunofluorescence
- double immunofluorescence terminal dUTP nick-end labeling (TUNEL)
- immunoblotting
Monoclonal anti-dystrophin antibody can be used for localization of dystrophin using immunochemical assays like ELISA and immunohistochemistry. The antibody can also be used in immunoblotting for brain dystrophin. Further, it can be used in western blotting and double immunofluorescent labelling (diluted 1: 500) of dystrophin.
Biochem/physiol Actions
Dystrophin deficiency is associated with severe Duchenne muscular dystrophy (DMD). Becker muscular dystrophy (BMD) show less pronounced abnormalities of dystrophin protein expression. Since abnormalities in the protein expression occur specifically in patients with these types of muscular dystrophy, dystrophin analysis may be used to distinguish these conditions from other neuromuscular diseases.
Other Notes
This product can be found as purified product that was produced using cell culture hybridoma product.
SAB4200764 Anti-Dystrophin antibody, Mouse monoclonal
clone MANDYS8, purified from hybridoma cell culture
SAB4200764 Anti-Dystrophin antibody, Mouse monoclonal
clone MANDYS8, purified from hybridoma cell culture
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Clase de almacenamiento
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Datasheet
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Rachel Blitzblau et al.
Brain research, 1218, 21-34 (2008-06-06)
Muscular dystrophy patients often show cognitive impairment, in addition to muscle degeneration caused by dystrophin gene defects. The cognitive impairments lead to speculation that the dystrophin protein family may play a key role at neuronal synapses. Dystrophin regulates the stability
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Kielbasa OM, et al.
Faseb Journal, 25(7), 2276-2286 (2011)