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Merck

D8515

Deoxyribonucleic acid−cellulose double-stranded from calf thymus DNA

lyophilized powder

Sinónimos:

DNA, Deoxyribonucleic acid, ds-DNA-Cellulose, DNA-Cellulose

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UNSPSC Code:
41106305
eCl@ss:
32160414
NACRES:
NA.51
MDL number:
Biological source:
bovine (calf) thymus
Form:
lyophilized powder
Storage temp.:
−20°C
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biological source

bovine (calf) thymus

Quality Level

form

lyophilized powder

extent of labeling

3-8 mg double-stranded calf thymus DNA/g

storage temp.

−20°C

General description

Deoxyribonucleic acid−cellulose double-stranded from calf thymus DNA, also known as ctDNA is a natural source of DNA designed for DNA binding agent studies. The detection limits of ctDNA are around 2.0ng and the linear ranges of light scattering intensity (I(LS)) is 0.05-1.5 mµg.

Application

Deoxyribonucleic acid−cellulose double-stranded from calf thymus DNA is used to study the binding parameters and binding specificity of a variety of proteins, nucleotides, and chemical reagents including anti-cancer agents. It is frequently used in pull-down assays and chromatographic procedures.

Analysis Note

May contain a trace of Tris and EDTA.

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Journal of immunology (Baltimore, Md. : 1950), 198(7), 2568-2577 (2017-02-22)
CD74 mediates MHC class-II antigenic peptide loading and presentation and plays an important role in the pathogenesis of autoimmune diseases, including systemic lupus erythematosus. C57BL/6
A Georgaki et al.
Nucleic acids research, 22(7), 1128-1134 (1994-04-11)
A DNA helicase from calf thymus, called DNA helicase F, copurified with replication protein A through several steps of purification including DEAE-Sephacel, hydroxyapatite and single stranded DNA cellulose. It is finally separated from replication protein A on FPLC Mono Q
Liyun Zhang et al.
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T Kuzuhara et al.
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