Anti-Mouse IgG (whole molecule)–FITC antibody produced in sheep

Immunoglobulin G (IgG) is available in large quantities in the human serum. It constitutes about 10-20% of the plasma proteins. IgG is a glycoprotein, with 82-96% proteins and 4-18% carbohydrates. It consists of four sub-classes i.e IgG1, IgG2, IgG3, and IgG4. IgG is composed of four polypeptide chains-two heavy chains (γ chains) and two light chains (κ or λ chains) which are linked by inter-chain disulfide bonds.

Anti-Mouse IgG (whole molecule)−FITC antibody produced in sheep has been used in:

• flow cytometry
• immunocytochemistry
• immunofluorescent staining

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of FITC to Anti- Mouse IgG (whole molecule) antibody allows for the visualization of protein by fluorescent microscopy.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Antibody adsorbed with human serum proteins.

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