F4049
ANTI-FLAG® M2 monoclonal antibody produced in mouse
clone M2, purified immunoglobulin, buffered aqueous solution
Sinónimos:
Anti-ddddk, Anti-dykddddk
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NACRES:
NA.32
UNSPSC Code:
12352203
Conjugate:
FITC conjugate
Clone:
M2, monoclonal
Application:
direct immunofluorescence
Species reactivity:
all
Citations:
114
Technique(s):
direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone
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Permítanos ayudarleNombre del producto
ANTI-FLAG® M2 monoclonal antibody produced in mouse, clone M2, purified immunoglobulin, buffered aqueous solution
biological source
mouse
conjugate
FITC conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
M2, monoclonal
form
buffered aqueous solution
species reactivity
all
concentration
~1 mg/mL
technique(s)
direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone
isotype
IgG1
immunogen sequence
DYKDDDDK
shipped in
dry ice
storage temp.
−20°C
Quality Level
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Application
Antibody is suitable for immunofluorescence, immunocytochemistry, immunohistochemistry, immunoblotting, flow cytometry and electron microscopy. Ideal for use when detection of Flag fusion proteins is required in a murine host, where secondary antibodies could cause cross reactivity.
Learn more product details in ourFLAG® application portal.
Learn more product details in ourFLAG® application portal.
Physical form
Solution in 10 mM phosphate buffered saline, plus 1% BSA and 0.1% sodium azide, pH 7.4
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
General description
Anti Flag M2-FITC antibody is used for the detection of Flag fusion proteins.This monoclonal antibody is covalently conjugated to flourescein isothiocyanate (FITC). The antibody recognizes the FLAG sequence at the N-terminus, Met N-terminus, and C-terminus.Binding is not Calcium dependent. F4049 is an Affinity purified antibody, increasing sensitivity in most applications.
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Clase de almacenamiento
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Molecular pharmacology, 71(6), 1487-1493 (2007-03-01)
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Journal of immunological methods, 362(1-2), 190-194 (2010-08-31)
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Xuechen Lv et al.
Protein & cell, 7(5), 325-337 (2016-04-18)
G protein-coupled receptors (GPCRs) are involved in all human physiological systems where they are responsible for transducing extracellular signals into cells. GPCRs signal in response to a diverse array of stimuli including light, hormones, and lipids, where these signals affect
Emmanuelle Despras et al.
Nature communications, 7, 13326-13326 (2016-11-05)
Translesion polymerase eta (polη) was characterized for its ability to replicate ultraviolet-induced DNA lesions that stall replicative polymerases, a process promoted by Rad18-dependent PCNA mono-ubiquitination. Recent findings have shown that polη also acts at intrinsically difficult to replicate sequences. However
Martina Podleschny et al.
PloS one, 10(12), e0145169-e0145169 (2015-12-19)
Neural crest cells are a highly migratory pluripotent cell population that generates a wide array of different cell types and failure in their migration can result in severe birth defects and malformation syndromes. Neural crest migration is controlled by various
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