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Merck

H7523

HEPES

BioXtra, pH 5.0-6.5 (1 M in H2O), ≥99.5% (titration)

Sinónimos:

Ácido 4-(2-hidroxietil)piperacina-1-etanosulfónico, Ácido N-(2-hidroxietil)piperacina-N′-(2-etanosulfónico)

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About This Item

Fórmula empírica (notación de Hill):
C8H18N2O4S
Número CAS:
Peso molecular:
238.30
UNSPSC Code:
12161700
NACRES:
NA.25
PubChem Substance ID:
EC Number:
230-907-9
Beilstein/REAXYS Number:
883043
MDL number:

Nombre del producto

HEPES, BioXtra, pH 5.0-6.5 (1 M in H2O), ≥99.5% (titration)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

product line

BioXtra

assay

≥99.5% (titration)

form

powder

impurities

Insoluble matter, passes filter test

ign. residue (900°C)

≤0.1% (as SO4)

loss

≤1.0% loss on drying, 110°C

pH

5.0-6.5 (1 M in H2O)

useful pH range

6.8-8.2

pKa (25 °C)

7.5

solubility

H2O: 1 M, clear, colorless

anion traces

chloride (Cl-): ≤0.05%

cation traces

Al: ≤0.0005%
As: ≤0.0001%
Ba: ≤0.0005%
Bi: ≤0.0005%
Ca: ≤0.001%
Cd: ≤0.0005%
Co: ≤0.0005%
Cr: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Li: ≤0.0005%
Mg: ≤0.0005%
Mn: ≤0.0005%
Mo: ≤0.0005%
Na: ≤0.005%
Ni: ≤0.0005%
Pb: ≤0.0005%
Sr: ≤0.0005%
Zn: ≤0.0005%

absorption

≤0.035 at 280 in H2O at 1 M
≤0.045 at 260 in H2O at 1 M

application(s)

diagnostic assay manufacturing

Quality Level

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Application

HEPES has been used:
  • As a component of homogenisation buffer used in protein sample preparation
  • To neutralise PRP (platelet-rich plasma) solution as part of PRP preparation
  • As a component in 2X PBS buffer, which is used in phosphate calcium transfection in cell line preparation
  • As a supplement in RPMI 1640 medium used to culture human umbilical vein endothelial cells (HUVEC) and in M199 complete medium for ex-vivo treatment of testicular tissue.

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Other Notes

Easily compare specifications for HEPES products with the HEPES specification table.

Clase de almacenamiento

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Ogilvie KM, et al.
Biology of Reproduction, 60(2), 527-533 (1999)
In vitro tubulogenesis of endothelial cells by relaxation of the coupling extracellular matrix-cytoskeleton.
Deroanne CF, et al.
Cardiovascular Research, 49(3), 647-658 (2001)
Hsp60-induced tolerance in the rotifer Brachionus plicatilis exposed to multiple environmental contaminants.
Wheelock CE, et al.
Archives of Environmental Contamination and Toxicology, 36(3), 281-287 (1999)
Intervertebral disc regeneration in an ex vivo culture system using mesenchymal stem cells and platelet-rich plasma.
Chen WH, et al.
Biomaterials, 30(29), 5523-5533 (2009)
Assessing the corrosion of biodegradable magnesium implants: a critical review of current methodologies and their limitations.
Kirkland NT, et al.
Acta Biomaterialia, 8(3), 925-936 (2012)

Contenido relacionado

HEPES Specification Comparison

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