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Merck

H8891

Heparinase III from Flavobacterium heparinum

Lyophilized powder stabilized with approx. 25% (w/w) bovine serum albumin, ≥30 units/mg protein (enzyme + BSA)

Sinónimos:

Heparin Lyase III, Heparitinase I, Heparitinase from Flavobacterium heparinum

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Número CAS:
37290-86-1
UNSPSC Code:
12352204
NACRES:
NA.54
Número CE:
4.2.2.8 (BRENDA, IUBMB)
MDL number:
MFCD00131319

Documentos clave

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Nombre del producto

Heparinase III from Flavobacterium heparinum, Lyophilized powder stabilized with approx. 25% (w/w) bovine serum albumin, ≥30 units/mg protein (enzyme + BSA)

biological source

bacterial (Flavobacterium heparinum)

conjugate

conjugate (Glucosaminoglycan)

form

lyophilized powder

specific activity

≥30 units/mg protein (enzyme + BSA)

mol wt

70.8 kDa

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

Quality Level

200

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Application

Heparinase III from Flavobacterium heparinum has been used to cleave specific GAGs. It has been used to study its effect on the electrophoretic mobility of the protein reacting with the anti-aFGF (acidic fibroblast growth factor) antibody.

Biochem/physiol Actions

Heparin-degrading lyase that recognizes heparin sulfate proteoglycan as its primary substrate.
Heparinase III prevents the formation of new blood vessels in vivo. It specifically degrades heparin sulphate at the HNY-I and HNY,6X-G2 links. Unlike other heparinise, heparinase III does not contain the amino acid cysteine. It contains 13 histidine residues, which is responsible for its catalytic action. This enzyme is not active towards heparin or low molecular weight heparins.

Other Notes

One unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25 °C. One International Unit (I.U.) is equivalent to approx. 600 Sigma units. Package sizes are sold in Sigma units.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000444454
0000444456
0000444456
0000444454
0000444458

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Is Product No. H8891, Heparinase III sold based on Sigma Units or International Units?

    This material is sold based on Sigma Units.  One Sigma Unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25 °C. One International Unit (I.U.) is equivalent to approx. 600 Sigma units.  

  6. Does Product No. H8891, Heparinase III, contain calcium?

    This product is lyophilized from a solution which is 75 mM NaCl/ 5mM NaH2PO4. We have no reason to believe that calcium ions are present.

  7. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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