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Merck

H9289

Anti-Histone H3 (N-terminal) antibody produced in mouse

affinity isolated antibody, buffered aqueous solution

Sinónimos:

Histone H3 Detection, Histone H3 Detection Antibody, Rabbit Anti-Histone H3

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NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
indirect immunofluorescence
western blot
Species reactivity:
human
Citations:
37
Technique(s):
indirect immunofluorescence: 2-4 μg/mL using A431 cells.
western blot: 0.2-0.5 μg/mL using PC12 cell lysate and A431 cell lysate.

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Nombre del producto

Anti-Histone H3 (N-terminal) antibody produced in mouse, affinity isolated antibody, buffered aqueous solution

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~17 kDa

species reactivity

human

enhanced validation

independent
Learn more about Antibody Enhanced Validation

technique(s)

indirect immunofluorescence: 2-4 μg/mL using A431 cells.
western blot: 0.2-0.5 μg/mL using PC12 cell lysate and A431 cell lysate.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... HIST1H3A(8350)

Categorías relacionadas

Application

Anti-histone H3 (N-terminal) antibody produced in rabbit has been used in western blotting and native chromatin immunoprecipitation assay.

Biochem/physiol Actions

Histones acts as structural scaffolds for DNA packaging. Histone H3 plays an important role in replication and DNA repair. Histone H3 is heavily post-translationally modified than the other members of histone family. Mutations in the gene leads to cerebellar high-grade gliomas.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Anti-Histone H3 (N-terminal) specifically recognizes human histone H3 (~17kDa). Histone H3/ histone cluster 1 H3 family member a (HIST1H3A) is a core member of the histone family. HIST1H3A is encoded by the gene mapped to human chromosome 6p22.2. The encoded protein is composed of ~136 amino acid.

Immunogen

synthetic peptide corresponding to amino acids 1-20 of human histone H3, conjugated to KLH.

Other Notes

In order to obtain the best results using various techniques and preparations, we recommend determining the optimal working dilutions by titration.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frostfree” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

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Clase de almacenamiento

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000440298
0000440298
0000399747
0000399747
0000298316

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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Phosphorylation of histone H3 Ser10 establishes a hierarchy
for subsequent intramolecular modification events
Liokatis S, et al.
Nature Structural and Molecular Biology, 19, 819-823 (2012)
Hua Jiang et al.
Genes & development, 31(12), 1272-1287 (2017-07-27)
Hybrid seed lethality as a consequence of interspecies or interploidy hybridizations is a major mechanism of reproductive isolation in plants. This mechanism is manifested in the endosperm, a dosage-sensitive tissue supporting embryo growth. Deregulated expression of imprinted genes such as
H3K9me3-binding proteins are dispensable for SETDB1/H3K9me3-dependent retroviral silencing
Maksakova IA, et al.
Epigenetics & Chromatin, 4 (2011)
Histone H3 K27M mutations in adult cerebellar high-grade gliomas.
Nakata S, et al.
Brain Tumor Pathology, 34, 113-119 (2017)
Rita A Batista et al.
eLife, 8 (2019-12-04)
MADS-box transcription factors (TFs) are ubiquitous in eukaryotic organisms and play major roles during plant development. Nevertheless, their function in seed development remains largely unknown. Here, we show that the imprinted Arabidopsis thaliana MADS-box TF PHERES1 (PHE1) is a master
Ver todos los artículos relacionados

Contenido relacionado

Data Sheet - H9289

Instructions

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