H9661
Hepatocyte Growth Factor human
≥95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture
Sinónimos:
HGF, Hepatopoietin A, Scatter Factor
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Biological source:
human
Recombinant:
expressed in NSO cells
Assay:
≥95% (SDS-PAGE)
Form:
lyophilized powder
Mol wt:
70-80 kDa by SDS-PAGE (non-reducing)
Impurities:
≤4 ng/mL (ED50)
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Permítanos ayudarleNombre del producto
Hepatocyte Growth Factor human, HGF, recombinant, expressed in NSO cells, suitable for cell culture
biological source
human
recombinant
expressed in NSO cells
assay
≥95% (SDS-PAGE)
form
lyophilized powder
potency
20-40 ng/mL
quality
endotoxin tested
mol wt
70-80 kDa by SDS-PAGE (non-reducing)
packaging
pkg of 5 and 25 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤4 ng/mL (ED50)
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
human ... HGF(3082)
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General description
Recombinant, human hepatocyte growth factor (HGF) is produced from the 728 amino acid residue variant of the human pre-pro-HGF protein 6 and expressed in a mouse myeloma cell line NSO.The HGF gene is mapped to human chromosome 7q21.1. The encoded protein is a mesenchymal-fibroblast derived cytokine.
Application
Hepatocyte Growth Factor human has been used as a growth factor cocktail to suspend rat fibrogen/thrombin cells. It has also been used to study the tyrosine phosphorylation of MET (MET proto-oncogene, receptor tyrosine kinase) in myxofibrosarcoma cell line.
Biochem/physiol Actions
HGF′s major function is to induce in vitro hepatocytes proliferation. HGF is also essential for cell migration and angiogenesis. Itis associated with embryonic development and tissue homeostasis. It is known to mediate placental trophoblast invasion, which is essential for normal fetal growth. Abnormal and defective HGF causes preeclampsia due to placental insufficiency. HGF exhibits its multifunctions through c-MET (proto-oncogene tyrosine kinase receptor). HGF is known to induce human umbilical vein endothelial cell proliferation and vasculogenic mimicry migration in gastric cancer cells, thus serving as a good target for cancer therapy. Upregulation of the HGF gene is observed in advanced stages of cancer, such as pancreatic adenocarcinoma.
Hepatocyte growth factor (HGF) is a potent mitogen for hepatocytes and a variety of cell types including endothelial and epithelial cells, melanocytes, and keratinocytes. It also mediates epithelial morphogenesis. HGF is the ligand for the tyrosine kinase receptor encoded by the met proto-oncogene.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 50 μg of bovine serum albumin per 1μg of cytokine.
Analysis Note
The biological activity is measured by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells.
Other Notes
The NSO-expressed recombinant HGF is greater than 95% heterodimeric HGF. The insect cell line-expressed recombinant HGF is a mixture of single chain HGF (predominant) with some heterodimeric HGF. Single chain HGF and heterodimeric HGF are equally active in in vitro assays.
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Dual inhibition of Hedgehog and c-Met pathways for pancreatic cancer treatment
Rucki AA, et al.
Molecular Cancer Therapeutics (2017)
Correlation between HGF/c-Met and Notch1 signaling pathways in human gastric cancer cells
Huang KH, et al.
Oncology Reports, 40(1), 294-302 (2018)
HGF derived from cancer-associated fibroblasts promotes vascularization in gastric cancer via PI3K/AKT and ERK1/2 signaling
Ding X, et al.
Oncology Reports (2018)
Analysis of hepatocyte growth factor immunostaining in the placenta of HIV-infected normotensive versus preeclamptic pregnant women
Cele SB,et al.
Eur. J. Obstet. Gyn. Rep. Biol. (2018)
Prognostic implication of MET overexpression in myxofibrosarcomas: an integrative array comparative genomic hybridization, real-time quantitative PCR, immunoblotting, and immunohistochemical analysis
Lee JC, et al.
Modern Pathology, 23(10), 1379-1379 (2010)
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