M7292
M16 Medium
With sodium bicarbonate and lactic acid, without penicillin and streptomycin, liquid, sterile-filtered
Sinónimos:
Cell Culture Medium, Cell Culture Medium M16, M16 Medium Solution
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NACRES:
NA.75
UNSPSC Code:
12352207
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Permítanos ayudarleNombre del producto
M16 Medium, With sodium bicarbonate and lactic acid, without penicillin and streptomycin, liquid, sterile-filtered
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | embryo: suitable
impurities
endotoxin, tested
components
NaHCO3: 2.101 g/L
phenol red: 0.0106 g/L
sodium pyruvate: 0.0363 g/L
glucose: 1.0 g/L (Dextro)
shipped in
ambient
storage temp.
2-8°C
Quality Level
Analysis Note
Presence of particulates in solution will not affect product′s performance.
This product is tested for its ability to support the development of one-cell mouse embryos to expanded blastocysts. Minimum requirement is 80% development to blastocyst.
Application
M2 and M16 Medium are common media for in vitro culture of preimplantation stage embryos. It is a modified Krebs-Ringer bicarbonate solution, which is very similar to Whitten′s Medium. M16 contains pyruvate and lactate as energy sources since preimplantation embryos do not utilize glucose efficiently.
Preparation Note
Supplement with 0.06 g/L potassium penicillin-G and 0.05 g/L streptomycin sulfate.
Clase de almacenamiento
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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