M8662
Aceite de inmersión
PCR Reagent
Sinónimos:
Mineral oil for PCR
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Acerca de este artículo
Technique(s):
PCR: suitable
Servicio técnico
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Permítanos ayudarleQuality Level
form
liquid
feature
PCR Additives
packaging
vial of 1.5 mL (Total volume 7.5 mL (5 vials))
technique(s)
PCR: suitable
color
colorless
refractive index
n20/D 1.467 (lit.)
density
0.84 g/mL at 25 °C (lit.)
foreign activity
DNase, RNase, protease, none detected
storage temp.
room temp
InChI key
AEOVEGJBKQQFOP-DDVLFWKVSA-L
General description
Mineral oil is used as a reaction mix overlay in polymerase chain reactions (PCR) to prevent reaction solutions from evaporating during the thermal cycling in PCR instruments without heated lids.
Application
Mineral oil has been used:
- for routine PCR amplifications
- to improve the heat conductivity between the device and the cycler during reverse transcription-polymerase chain reaction (RT–PCR) amplification
- to prevent evaporation during single-cell MATQ-sequencing performed on Bravo automated liquid handling platform
Features and Benefits
- Provided in a convenient 5 × 1.5 mL (1 vial) pack size
- Tested for the absence of DNase, RNase, and protease.
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Clase de almacenamiento
10 - Combustible liquids
wgk
WGK 1
flash_point_f
No data available
flash_point_c
No data available
ppe
Eyeshields, Gloves
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Contenido relacionado
Instructions
Hsin-I Jen et al.
eLife, 8 (2019-04-30)
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Nature communications, 5, 3451-3451 (2014-03-29)
Discriminating cellular heterogeneity is important for understanding cellular physiology. However, it is limited by the technical difficulties of single-cell measurements. Here we develop a two-stage system to determine cellular heterogeneity. In the first stage, we perform multiplex single-cell RNA cytometry
Decagram scale production of deuterated mineral oil and polydecene as solvents for polymer studies in neutron scattering
Klenner MA, et al.
Open Journal of Polymer Chemistry, 11(31), 4986-4994 (2020)