N7653
p-Nitrophenyl Phosphate Liquid Substrate System
alkaline phosphatase substrate, liquid
Sinónimos:
4-Nitrophenyl phosphate disodium salt solution, pNPP
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Fórmula lineal:
O2NC6H4OP(O)(ONa)2
Número CAS:
Peso molecular:
263.05
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.83
MDL number:
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Permítanos ayudarleNombre del producto
p-Nitrophenyl Phosphate Liquid Substrate System, liquid
SMILES string
[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
InChI
1S/C6H6NO6P.2Na/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;/h1-4H,(H2,10,11,12);;/q;2*+1/p-2
InChI key
VIYFPAMJCJLZKD-UHFFFAOYSA-L
form
liquid
shipped in
wet ice
storage temp.
−20°C
Quality Level
Categorías relacionadas
General description
The p-Nitrophenyl Phosphate (pNPP) Liquid Substrate System combines p-nitrophenyl phosphate, buffer, and the required magnesium cations in a convenient, ready-to-use, single solution reagent. Producing a soluble end product, pNPP is the substrate of choice in alkaline phosphatase enzyme immunoassays.
Application
p-Nitrophenyl Phosphate Liquid Substrate System has been used as a substrate to determine the activity of alkaline phosphatase in alkaline phosphatase assay.
Substrate for alkaline phosphatase that yields a soluble yellow end product that may be read at 405 nm. The reaction may be stopped with 3N NaOH and read at 405 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.
Biochem/physiol Actions
4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP produces p-nitrophenol. It is a common chromogenic substrate for alkaline phosphatase.
Physical form
Ready-to-use.
Disclaimer
Stable at least one year when stored below 0 °C.
signalword
Danger
Hazard Classifications
Eye Dam. 1 - Met. Corr. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral
target_organs
Kidney,Liver,Blood
Clase de almacenamiento
12 - Non Combustible Liquids
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Journal of immunological methods, 118(1), 79-84 (1989-03-10)
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