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Merck

P1644

Percoll®

pH 8.5-9.5 (20 °C)

Sinónimos:

Medios de gradiente de densidad Percoll

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD00131830

Nombre del producto

Percoll®, pH 8.5-9.5 (20 °C)

sterility

aseptically filled

form

colloidal ((Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)) )

technique(s)

cell culture | mammalian: suitable

color

clear colorless to faint yellow

pH

8.5-9.5 (20 °C)

application(s)

hematology
histology

storage temp.

2-8°C

Quality Level

200

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Application

El Percoll consiste en partículas de sílice coloidales de 15 a 30 nm de diámetro 23 % p/p en agua) que han sido recubiertas con polivinilpirrolidona (PVP. Se utiliza en la separación celular y en el aislamiento de orgánulos.
El Percoll consiste en partículas de sílice coloidales de 15 a 30 nm de diámetro 23 % p/p en agua) que han sido recubiertas con polivinilpirrolidona (PVP. Se utiliza para establecer gradientes de densidad no tóxicos con baja viscosidad y poca osmolaridad que pueden utilizarse para aislar células, orgánulos y virus. Se ha utilizado para separar vesículas de la membrana interna y externa de E. coli. El Percoll es isosmótico en todo el gradiente, no tóxico y fácil de eliminar de los materiales purificados.
Se ha utilizado Percoll® para la preparación del esperma en estudios de los requisitos de energía de los embriones bovinos.

General description

Percoll® es un medio clásico para centrifugación en gradiente de densidad de células, virus y partículas celulares. Percoll consiste en partículas de sílice coloidal de 15– 30 nm de diámetro (23% p/p en agua) que han sido recubiertas con polivinilpirrolidona (PVP). La PVP hace que el producto sea completamente atóxico e ideal para utilizar con materiales biológicos.

Legal Information

Percoll is a registered trademark of Cytiva

Clase de almacenamiento

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

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Certificados de análisis (COA)

Lot/Batch Number
0000517841
0000517841
0000501899
0000501899
0000511507

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  1. Can you help me calculate gradients for Percoll®, Product P1644?

    The product information sheet (under Documents, above) has an equation for calculating density of a given solution.

  2. When using Product P1644, Percoll®, what density should I use for my particular system?

    The product information sheet (under Documents, above) has a table of densities for different organelles and cell types.

  3. Is there a "best" salt solution to use with Product P1644, Percoll®?

    Percoll can be used with various salt formulations (PBS, 1.5 M NaCl, HBSS, etc.). Physiological saline and balanced salt solutions can be suggested.  Sucrose is included to prevent aggregation.  You can find detailed preparation instructions on the product information sheet (under Documents, above) for this product.

  4. Can Percoll®, Product P1644, be autoclaved?

    Percoll can be autoclaved, but this must be done without any salts or sucrose present.

  5. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  6. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. What is the difference between Percoll® Products P4937 and P1644?

    Product Nos. P1644 and P4937 are both Percoll. Product No. P4937 has additional testing for use in cell culture.

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Tanja Zöller et al.
International journal of molecular sciences, 19(3) (2018-03-02)
Microglia are the resident immune cells of the central nervous system (CNS) and participate in physiological and pathological processes. Their unique developmental nature suggests age-dependent structural and functional impairments that might contribute to neurodegenerative diseases. In the present study, we
R Hovius et al.
Biochimica et biophysica acta, 1021(2), 217-226 (1990-01-29)
Rat liver mitochondria were isolated by a combination of differential and Percoll gradient centrifugation, resulting in a highly pure and intact preparation, as assessed by marker enzyme analysis, latency of cytochrome-c oxidase, respiratory control index and electron microscopy. Two different
Noa Bossel Ben-Moshe et al.
Nature communications, 10(1), 3266-3266 (2019-07-25)
Complex interactions between different host immune cell types can determine the outcome of pathogen infections. Advances in single cell RNA-sequencing (scRNA-seq) allow probing of these immune interactions, such as cell-type compositions, which are then interpreted by deconvolution algorithms using bulk
Development of bovine embryos in vitro as affected by energy substrates.
Rosenkrans Jr C F, et al.
Biology of Reproduction, 49(3), 459-462 (1993)
Guilherme D Melo et al.
Scientific reports, 7(1), 8454-8454 (2017-08-18)
Visceral leishmaniasis (VL) is a systemic disease with multifaceted clinical manifestations, including neurological signs, however, the involvement of the nervous system during VL is underestimated. Accordingly, we investigated both brain infection and inflammation in a mouse model of VL. Using
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