P2922
Endoproteinase Glu-C from Staphylococcus aureus V8
Type XVII-B, lyophilized powder, 500-1,000 units/mg solid
Sinónimos:
V8 Protease
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Número CAS:
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54
MDL number:
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Endoproteinase Glu-C from Staphylococcus aureus V8, Type XVII-B, lyophilized powder, 500-1,000 units/mg solid
type
Type XVII-B
form
lyophilized powder
specific activity
500-1,000 units/mg solid
mol wt
29 kDa
purified by
chromatography
shipped in
wet ice
storage temp.
−20°C
Quality Level
Gene Information
Staphylococcus aureus subsp. aureus MW2 ... sspA(1003044)
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Application
Endoproteinase Glu-C from Staphylococcus aureus strain V8 is a serine protease used for selective cleavage of proteins for amino acid sequence determination or peptide mapping . Product P2922 has been used to linearize cyclic peptides in C. ternatea leaf extract.
Endoproteinase Glu-C from Staphylococcus aureus V8 has been used to digest reduced and alkylated cyclotides to produce linearized fragments.
It is used for selective cleavage of proteins for amino acid sequence determination or peptide mapping.
Biochem/physiol Actions
Staphylococcus strain V8 protease specifically cleaves peptide bonds on the carboxyl side of aspartic and glutamic acid residues when used in phosphate buffer. When used in ammonium bicarbonate buffer or ammonium acetate buffer cleavage is restricted to the carboxyl side of glutamic acid residues only. The enzyme exhibits maximal activity from pH 4.0 to 7.8. If hemoglobin is used as the substrate, maximal activity is at pH 4.0. The maximal activity is at pH of 7.8 when casein is the substrate.
Other Notes
One unit will hydrolyze 1 μmole of N-t-Boc-L-glutamic acid α-phenyl ester per min at pH 7.8 at 37 °C. One unit is equivalent to ~0.004 casein digestion unit.
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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