P3243
Phosphodiesterase I from Crotalus adamanteus venom
vial of ≥100 units, Purified
Sinónimos:
5′-Exonuclease, Oligonucleate 5′-nucleotidohydrolase
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About This Item
Número CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-806-5
MDL number:
Nombre del producto
Phosphodiesterase I from Crotalus adamanteus venom, vial of ≥100 units, Purified
biological source
Crotalus adamanteus venom
form
solid
quality
Purified
specific activity
≥20.0 unit/mg solid
packaging
vial of ≥100 units
storage temp.
−20°C
Quality Level
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Application
Phosphodiesterase (PDE) is any enzyme that is used to breaks phosphodiester bonds. It is a membrane-bound glycoprotein that is used to catalyze the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is used in phosphodiesterase activation assays to hydrolyze AMP. Product P3243 is from Crotalus adamanteus venom and is purified. Product P3243 has been used to hydrolyze tRNA with wyosine derivatives into mononucleosides.
Biochem/physiol Actions
Phosphodiesterase I breaks phosphodiester bonds and catalyzes the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is released from eucaryotic plasma membranes by phosphatidylinositol-specific phospholipase C.
Other Notes
One Unit hydrolyzes one μmole of p-nitrophenyl thymidine-5-phosphate per minute at 25 °C, pH 8.9
Preparation Note
For testing purposes, PDE I is reconstituted in cold deionized water at 0.1 - 0,2 un/mL.
Purified via the method of Williams, et al. and further treated to inactivate contaminating 5′-nucleotidase activity.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Clase de almacenamiento
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Valérie de Crécy-Lagard et al.
Molecular biology and evolution, 27(9), 2062-2077 (2010-04-13)
Wyosine (imG) and its derivatives such as wybutosine (yW) are found at position 37 of phenylalanine-specific transfer RNA (tRNA(Phe)), 3' adjacent to the anticodon in Eucarya and Archaea. In Saccharomyces cerevisiae, formation of yW requires five enzymes acting in a
Action of venom phosphodiesterase on deoxyribonucleic acid.
E J WILLIAMS et al.
The Journal of biological chemistry, 236, 1130-1134 (1961-04-01)
N Shenoy et al.
Blood cancer journal, 7(7), e587-e587 (2017-07-22)
The Ten Eleven Translocation (TET) enzymes have been found to be mutated in both diffuse large B-cell (DLBCL) and peripheral T-cell (PTCL) lymphomas resulting in DNA hypermethylation. Recent studies in embryonal stem cells showed that ascorbic acid (AA) is a
Yana Konokhova et al.
Skeletal muscle, 6, 10-10 (2016-02-20)
Low mitochondrial content and oxidative capacity are well-established features of locomotor muscle dysfunction, a prevalent and debilitating systemic occurrence in patients with chronic obstructive pulmonary disease (COPD). Although the exact cause is not firmly established, physical inactivity and oxidative stress
Jongwon Lim et al.
ACS omega, 5(23), 14173-14179 (2020-06-23)
The metazoan second messenger 2'3'-cyclic guanosine monophosphate-adenosine monophosphate (cGAMP) is a cyclic dinucleotide (CDN) that induces secretion of type I interferons and activates the immune system and has thus attracted significant interest as a vaccine adjuvant or immunotherapeutic. CDN bisphosphorothioates
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