P4557
Fenol solution
Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, BioReagent, Molecular Biology
Sinónimos:
Tris-equilabrated Phenol
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About This Item
Nombre del producto
Fenol solution, Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, BioReagent, Molecular Biology
InChI key
ISWSIDIOOBJBQZ-UHFFFAOYSA-N
InChI
1S/C6H6O/c7-6-4-2-1-3-5-6/h1-5,7H
SMILES string
Oc1ccccc1
grade
Molecular Biology
product line
BioReagent
assay
≥99.0% (GC)
form
liquid
dilution
(for analytical testing)
pH
7.7-8.1 (25 °C)
storage temp.
2-8°C
Quality Level
Gene Information
human ... GABRA1(2554)
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Application
- como componente de la mezcla de reacción de digestión para preparar plásmidos sin ARNasas destinados a la traducción in vitro en un lisado de reticulocitos de conejo
- para la extracción de ADN de hojas de roseta Arabidopsis thaliana
- en la extracción de ADN de sangre en papel conservante por protocolo estándar de fenol-cloroformo
- como componente del tampón de extracción para extraer ARN de la pulpa de la fruta
- para la extracción de proteínas de las raíces
- para el aislamiento del ADN fecal
- para la extracción de ARN enjaulado
Biochem/physiol Actions
General description
Other Notes
- 1 botella de fenol equilibrado con Tris (referencia P4557)
- Tampón de equilibrio (referencia B5658)
signalword
Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
target_organs
Nervous system,Kidney,Liver,Skin
Clase de almacenamiento
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
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Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What is the molarity of Phenol solution, Product P4557?
The Molarity is ~10.6. The density of the phenol is 1.05g/ml, and the molecular weight is 94.11.
I see that there are two layers in the bottle Phenol solution, Product P4557. Which layer do I use?
The top phase is Tris buffer, so use the lower/bottom phase.
What do I do to equilibrate the Phenol solution, Product P4557, to around pH 8?
This phenol solution (Product No. P4557) includes a separate bottle of equilibration buffer (Product No. B5658) to adjust the phenol phase to pH 7.9±0.2. After addition of the buffer to the phenol, it may take about 6 hours (mixture can be kept at 2-8 °C) until the bottom phase (phenol) becomes clear. Some of the haziness in the phenol phase may be due to some air bubbles caused by mixing by inversion. There could be a hazy interface between the phenol and aqueous phase which remains after the separation. The entire contents of the bottle of the buffer should be added to the phenol. If buffering less than the complete bottle, use at a ratio of 6.5 mL buffer to 100 mL phenol. For 10 mL of phenol, use 650 μL of buffer. For 1 mL of phenol, use 65 μL of buffer.
What is the final pH of Phenol solution, Product P4557, after adding the buffer?
The pH is typically 8.0; the specifications state a range of 7.7-8.1.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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