P5282
Phalloidin Peptide
≥90% (HPLC), solid, FITC labeled
Sinónimos:
Phalloidin-FITC
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Fórmula empírica (notación de Hill):
C56H60N10O15S2
Peso molecular:
1177.26
UNSPSC Code:
12352202
NACRES:
NA.32
MDL number:
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Permítanos ayudarleNombre del producto
Phalloidin, Fluorescein Isothiocyanate Labeled, sequence Amanita phalloides(synthetic: peptide sequence)
biological source
sequence from Amanita phalloides (synthetic: peptide sequence)
form
solid
fluorescence
λex 495 nm; λem 520 nm(lit.)
storage temp.
−20°C
Quality Level
Categorías relacionadas
General description
Phalloidin is a phallotoxin produced by death cap mushroom Amanita phalloides. It is a cyclic peptide, which interacts with actin and this was first identified in phalloidin-poisoned rats. It is a heptapeptide, cyclic in nature, with a crosslink between tryptophan at position 6 and cysteine at position 3. The side chain of amino acid 7 (γ-δ-dihydroxyleucine) in phalloidin, is accessible to modifications, through which fluorescently labelled phalloidin compounds can be produced.
Application
Phalloidin, Fluorescein Isothiocyanate Labeled has been used:
- To visualize F-Actin reorganization in primary neonatal cardiomyocytes (PNCMs) and H9C2 cells (rat cardiac myoblasts) following endothelin-1 (ET-1) and angiotensin II (Ang II) treatment.
- In immunochemistry to label microfilament.
- In immunofluorescence analysis to stain F-actin.
Biochem/physiol Actions
Toxin that binds polymeric F actin, stabilizing it and interfering with the function of actin-rich structures.
Phalloidin interacts with polymeric actin, and not oligomeric or monomeric forms. This interaction leads to highly stabilized actin filaments, which resist depolymerization and disassembly. In rats, this toxin causes death due to liver hemorrhage, and cells show abnormal actin clustering. The affinity of phalloidin to actin is not significantly altered after derivatizing fluorescently labelled phalloidin compounds. These compounds can be used to study actin structure and organization within eukaryotic cells.
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 2 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral
Clase de almacenamiento
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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